Journalartikel

Jahr der Veröffentlichung: 2002

Seiten: 1966-1973

Zeitschrift: Journal of Bacteriology

Bandnummer: 184

Heftnummer: 7

ISSN: 0021-9193

DOI Link: https://doi.org/10.1128/JB.184.7.1966-1973.2002

Verlag: American Society for Microbiology

Abstract: 
The sol operon of Clostridium acetobutylicum is the essential transcription unit for formation of the solvents butanol and acetone. The recent proposal that transcriptional regulation of this operon is controlled by the repressor Orf5/SoIR (R. V. Nair, E. NI. Green, D. E. Watson, G. N. Bennett, and E. T. Papoutsakis, J. Bacteriol. 181:319-330, 1999) was found to be incorrect. Instead, regulation depends on activation, most probably by the multivalent transcription factor SpoOA. The operon is transcribed from a single promoter. A second signal identified in primer extension studies results from mRNA processing and can be observed only in the natural host, not in a heterologous host. The first structural gene in the operon (adhE, encoding a bifunctional butyraldehyde/butanol dehydrogenase) is translated into two different proteins, the mature AdhE enzyme and the separate butanol dehydrogenase domain. The promoter of the sol operon is preceded by three imperfect repeats and a putative SpoOA-binding motif, which partially overlaps with repeat 3 (113). Reporter gene analysis performed with the lacZ gene of Thermoanaerobacterium thermosulfurigenes and targeted mutations of the regulatory region revealed that the putative SpoOA-binding motif, R3, and R1 are essential for control. The data obtained also indicate that an additional activator protein is involved.

Harvard-Zitierstil: Thormann, K., Feustel, L., Lorenz, K., Nakotte, S. and Dürre, P. (2002) Control of butanol formation in Clostridium acetobutylicum by transcriptional activation, Journal of Bacteriology, 184(7), pp. 1966-1973. https://doi.org/10.1128/JB.184.7.1966-1973.2002

APA-Zitierstil: Thormann, K., Feustel, L., Lorenz, K., Nakotte, S., & Dürre, P. (2002). Control of butanol formation in Clostridium acetobutylicum by transcriptional activation. Journal of Bacteriology. 184(7), 1966-1973. https://doi.org/10.1128/JB.184.7.1966-1973.2002