Journalartikel

Jahr der Veröffentlichung: 2010

Seiten: 41665-41674

Zeitschrift: Journal of Biological Chemistry

Bandnummer: 285

Heftnummer: 53

ISSN: 0021-9258

DOI Link: https://doi.org/10.1074/jbc.M110.193292

Verlag: Elsevier

Abstract: 
Multiple DNA-associated processes such as DNA repair, replication, and recombination are crucial for the maintenance of genome integrity. Here, we show a novel interaction between the transcription elongation factor Bur1-Bur2 and replication protein A (RPA), the eukaryotic single-stranded DNA-binding protein with functions in DNA repair, recombination, and replication. Bur1 interacted via its C-terminal domain with RPA, and bur1-ΔC mutants showed a deregulated DNA damage response accompanied by increased sensitivity to DNA damage and replication stress as well as increased levels of persisting Rad52 foci. Interestingly, the DNA damage sensitivity of an rfa1 mutant was suppressed by bur1 mutation, further underscoring a functional link between these two protein complexes. The transcription elongation factor Bur1-Bur2 interacts with RPA and maintains genome integrity during DNA replication stress.

Harvard-Zitierstil: Clausing, E., Mayer, A., Chanarat, S., Müller, B., Germann, S., Cramer, P., et al. (2010) The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress, Journal of Biological Chemistry, 285(53), pp. 41665-41674. https://doi.org/10.1074/jbc.M110.193292

APA-Zitierstil: Clausing, E., Mayer, A., Chanarat, S., Müller, B., Germann, S., Cramer, P., Lisby, M., & Sträßer, K. (2010). The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress. Journal of Biological Chemistry. 285(53), 41665-41674. https://doi.org/10.1074/jbc.M110.193292