Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export - Research portal of Justus Liebig University Giessen:

Journal article

Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export

Authors list: Sträßer, K; Hurt, E

Publication year: 2000

Pages: 410-420

Journal: The EMBO Journal

Volume number: 19

Issue number: 3

ISSN: 0261-4189

DOI Link: https://doi.org/10.1093/emboj/19.3.410

Publisher: EMBO Press

Abstract:
Mex67p and Mtr2p constitute an essential mRNA export complex that interacts with poly(A)+ RNA and nuclear pore proteins. We have identified Yra1p, an intranuclear protein with in vitro RNA-RNA annealing activity, which directly binds to Mex67p. The complex between Yra1p and Mex67p was reconstituted in vitro and shown by UV-crosslinking to bind directly to RNA. Mutants of YRA1 are impaired in nuclear poly(A)+ RNA export at restrictive growth conditions. ALY, the mouse homologue of Yra1p and a transcriptional coactivator, can bind in vitro to yeast and human Mex67p and partly complements the otherwise non-viable yra1 null mutant. Thus, Yra1p is the first RNA-binding protein characterized, which bridges the shuttling Mex67p/Mtr2p exporter to intranuclear mRNA transport cargoes.

Authors/Editors

- Uni.-Prof. Dr. Katja Sträßer

Citation Styles

Harvard Citation style: Sträßer, K. and Hurt, E. (2000) Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export, The EMBO Journal, 19(3), pp. 410-420. https://doi.org/10.1093/emboj/19.3.410

APA Citation style: Sträßer, K., & Hurt, E. (2000). Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export. The EMBO Journal. 19(3), 410-420. https://doi.org/10.1093/emboj/19.3.410