Journal article

Publication year: 2009

Pages: 921-924

Journal: Journal of Ornithology

Volume number: 150

Issue number: 4

ISSN: 0021-8375

DOI Link: https://doi.org/10.1007/s10336-009-0423-1

Publisher: Springer

Abstract: 
We isolated seven novel polymorphic microsatellite DNA loci from the burrowing parrot (Cyanoliseus patagonus) and optimised them for future studies of population differentiation and genetic variation. The loci were screened for polymorphism using 38 samples from wild individuals from three neighbouring colonies in Argentina. The primers amplified highly variable loci characterised by 3-10 alleles per locus and their observed and expected heterozygosities ranged from 0.15 to 0.78 and 0.15 to 0.81, respectively. When we analysed 52 samples across Argentina and Chile, we found strong genetic differentiation between the Chilean and the Argentinean subspecies as well as significant differentiation between two geographically separated subspecies within Argentina. Our results indicate the suitability of these microsatellites for investigating further questions regarding the population genetics in this species.

Harvard Citation style: Klauke, N., Masello, J., Quillfeldt, P. and Segelbacher, G. (2009) Isolation of tetranucleotide microsatellite loci in the burrowing parrot (Cyanoliseus patagonus), Journal of Ornithology, 150(4), pp. 921-924. https://doi.org/10.1007/s10336-009-0423-1

APA Citation style: Klauke, N., Masello, J., Quillfeldt, P., & Segelbacher, G. (2009). Isolation of tetranucleotide microsatellite loci in the burrowing parrot (Cyanoliseus patagonus). Journal of Ornithology. 150(4), 921-924. https://doi.org/10.1007/s10336-009-0423-1