Journal article

Publication year: 2010

Pages: 2853-2865

Journal: Analytical and Bioanalytical Chemistry

Volume number: 398

Issue number: 7-8

ISSN: 1618-2642

DOI Link: https://doi.org/10.1007/s00216-010-4131-8

Publisher: Springer

Abstract: 
Novel peptides were identified in the skin secretion of the tree frog Hyla savignyi. Skin secretions were collected by mild electrical stimulation. Peptides were separated by reversed-phase high-performance liquid chromatography. Mass spectra were acquired by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS), and fragment ion spectra were obtained after collision-induced dissociation and electron capture dissociation. Peptides were analyzed by manual de novo sequencing and composition-based sequencing (CBS). Sequence analyses of three so far undescribed, structurally unrelated peptides are presented in this paper, having the sequences DDSEEEEVE-OH, P*EEVEEERJK-OH, and GJJDPJTGJVGGJJ-NH(2). The glutamate-rich sequences are assumed to be acidic spacer peptides of the prepropeptide. One of these peptides contains the modified amino acid hydroxyproline, as identified and localized by high-accuracy FTICR-MS. Combination of CBS and of experience-based manual sequence analysis as complementary and database-independent sequencing strategies resulted in peptide identification with high reliability.

Harvard Citation style: Langsdorf, M., Ghassempour, A., Römpp, A. and Spengler, B. (2010) Isolation and sequence analysis of peptides from the skin secretion of the Middle East tree frog Hyla savignyi, Analytical and Bioanalytical Chemistry, 398(7-8), pp. 2853-2865. https://doi.org/10.1007/s00216-010-4131-8

APA Citation style: Langsdorf, M., Ghassempour, A., Römpp, A., & Spengler, B. (2010). Isolation and sequence analysis of peptides from the skin secretion of the Middle East tree frog Hyla savignyi. Analytical and Bioanalytical Chemistry. 398(7-8), 2853-2865. https://doi.org/10.1007/s00216-010-4131-8