Peptide and protein identification by matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source decay time-of-flight mass spectrometry - Forschungsportal der Justus-Liebig-Universität Gießen:

Journalartikel

Peptide and protein identification by matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source decay time-of-flight mass spectrometry

Autorenliste: Chaurand, P; Luetzenkirchen, F; Spengler, B

Jahr der Veröffentlichung: 1999

Seiten: 91-103

Zeitschrift: Journal of The American Society for Mass Spectrometry

Bandnummer: 10

Heftnummer: 2

ISSN: 1044-0305

DOI Link: https://doi.org/10.1016/S1044-0305(98)00145-7

Verlag: American Chemical Society

Abstract:
The potential of matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source decay (PSD) time-of-flight mass spectrometry for the characterization of peptides and proteins is discussed. Recent instrumental developments provide for levels of sensitivity and accuracy that make these techniques major analytical tools for proteome analysis. New software developments employing protein database searches have greatly enhanced the fields of application of MALDI-PSD. Peptides and proteins can be easily identified even if only a partial sequence information is determined. Derivatization procedures have been optimized for MALDI-PSD to increase the structural information and to obtain a complete peptide sequence even in critical cases. They are fast, simple and can be performed on target. MALDI-PSD is also a very powerful tool to characterize or elucidate post-translational or chemically induced modifications. Ln association with database searches, proteins issued from electrophoretic gels can be identified after specific enzymatic cleavages and peptide mapping.

Zitierstile

Harvard-Zitierstil: Chaurand, P., Luetzenkirchen, F. and Spengler, B. (1999) Peptide and protein identification by matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source decay time-of-flight mass spectrometry, Journal of The American Society for Mass Spectrometry, 10(2), pp. 91-103. https://doi.org/10.1016/S1044-0305(98)00145-7

APA-Zitierstil: Chaurand, P., Luetzenkirchen, F., & Spengler, B. (1999). Peptide and protein identification by matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source decay time-of-flight mass spectrometry. Journal of The American Society for Mass Spectrometry. 10(2), 91-103. https://doi.org/10.1016/S1044-0305(98)00145-7