Journalartikel

Ligninolytic enzyme activities alternate with mushroom production during industrial cultivation of Pleurotus ostreatus on wheat straw-based substrate


AutorenlisteRühl, M; Fischer, C; Kües, U

Jahr der Veröffentlichung2008

Seiten478-492

ZeitschriftCurrent trends in biotechnology and pharmacy

Bandnummer2

Heftnummer4

URLhttp://www.abap.co.in/ligninolytic-enzyme-activities-alternate-mushroom-production-during-industrial-cultivation-pleurotus

VerlagAssociation of Biotechnology and Pharmacy (ABAP)


Abstract

Two commercial Pleurotus ostreatus strains (030 and K12) were cultivated
under industrial conditions on wheat straw blocks 17-18 kg in weight.
Within 10 weeks of cultivation, three flushes of fruiting body
production were obtained with total yields of 4.2 and 4.1 kg
mushrooms/substrate block and a biological efficiency (BE = total yield
of fresh mushrooms per dry weight of substrate) of about 95%,
respectively. Ligninolytic activities were followed up within the
substrate over the cultivation periods. Laccase, manganese peroxidase
(MnP) and versatile (manganese-independent) peroxidase (VP) activities
were high in vegetative growth phases within the substrate and dropped
upon initiation of fruiting body formation to be hardly detectable at
the harvest of the mature mushrooms. Directly after harvest at all
observed flushes, there was a sharp increase in all three enzymatic
activities in the substrate for both strains (maximum activities for
laccase 1.1-1.3 U/ml, for MnP 15-16 U/ml and for VP 1.1-1.4 U/ml).
Enzymes can be extracted by pressing the spent mushroom substrates
(SMS). Potential applications for the press juice or the enzymes in the
SMS are discussed.




Zitierstile

Harvard-ZitierstilRühl, M., Fischer, C. and Kües, U. (2008) Ligninolytic enzyme activities alternate with mushroom production during industrial cultivation of Pleurotus ostreatus on wheat straw-based substrate, Current trends in biotechnology and pharmacy, 2(4), pp. 478-492. http://www.abap.co.in/ligninolytic-enzyme-activities-alternate-mushroom-production-during-industrial-cultivation-pleurotus

APA-ZitierstilRühl, M., Fischer, C., & Kües, U. (2008). Ligninolytic enzyme activities alternate with mushroom production during industrial cultivation of Pleurotus ostreatus on wheat straw-based substrate. Current trends in biotechnology and pharmacy. 2(4), 478-492. http://www.abap.co.in/ligninolytic-enzyme-activities-alternate-mushroom-production-during-industrial-cultivation-pleurotus


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