Journal article

Publication year: 1994

Pages: 179-192

Journal: FEMS Microbiology Ecology

Volume number: 15

Issue number: 1-2

DOI Link: https://doi.org/10.1111/j.1574-6941.1994.tb00242.x

Publisher: Oxford University Press

Abstract: 

Molecular methods and conventional plating were applied to monitor Enterobacter agglomerans 339 derivatives carrying a Tn5‐Mob or an nptI‐cassette
in unsterile soil microcosms. The plate counts of the introduced
bacteria decreased continuously in time until undetectable on selective
media. In contrast, hybridization of the total DNA directly isolated
from inoculated soil samples showed that the target sequences detected
corresponded to a much higher number of bacteria than indicated by
plating. By PCR‐amplification and hybridization of the soil DNA we could
show that asignificant number of target sequences still persisted in
the soil microcosms, even when the inoculated bacteria were not able to
make colonies on selective agar plates. The Tn5 marker caused
instabilities in the genome of the bacteria studied. Some of the clones
that grew in the soil samples had rearrangements in their genome. The
detection of E. agglomerans 339 derivatives carrying the immobile nptI‐cassette was also dependent on its location in the bacterial genome.

Harvard Citation style: Evguenieva-Hackenberg, E., Selenska-Pobell, S. and Klingmüller, W. (1994) Persistence and stability of genetically manipulated derivatives of Enterobacter agglomerans in soil microcosms, FEMS Microbiology Ecology, 15(1-2), pp. 179-192. https://doi.org/10.1111/j.1574-6941.1994.tb00242.x

APA Citation style: Evguenieva-Hackenberg, E., Selenska-Pobell, S., & Klingmüller, W. (1994). Persistence and stability of genetically manipulated derivatives of Enterobacter agglomerans in soil microcosms. FEMS Microbiology Ecology. 15(1-2), 179-192. https://doi.org/10.1111/j.1574-6941.1994.tb00242.x