Journalartikel

Jahr der Veröffentlichung: 1986

Seiten: 336-345

Zeitschrift: Immunobiology

Bandnummer: 172

Heftnummer: 3-5

ISSN: 0171-2985

DOI Link: https://doi.org/10.1016/S0171-2985(86)80115-2

Verlag: Elsevier

Abstract: 
Murine interleukin 1 (IL 1) inhibited concentration dependently the proliferation of murine T cell lymphomas and the human leukemic cell line K 562. The cytostatic action of IL 1 was not associated with cytotoxicity and appeared to be irreversible. Changes in the expression of surface antigens, like a rapid decrease of transferrin receptors or, more delayed, an increase in HLA-A, B, C antigen density suggested that a differentiation step was induced by IL 1. This effect of IL 1 was a direct one and most likely mediated by a specific receptor molecule.
In order to characterize the receptor for IL 1, highly purified plasma membranes from K 562 were incubated with murine IL 1, and the phosphorylation pattern of plasma membrane proteins was investigated by the addition of radiolabeled ATP. At 0°C, IL 1 induced the specific phosphorylation of a 41 kDa membrane protein in a time- and concentrationdependent manner. Analysis of the phosphoamino acid composition revealed that IL 1 induced specifically the phosphorylation of tyrosine residues of the 41 kDa protein. Crosslinking experiments proved that the 41 kDa protein had an IL 1 binding site, strongly suggesting that the 41 kDa protein was the receptor for IL 1 itself. Affinity labeling with an ATP-analogue showed that this protein possessed an ATP binding and cleaving site.
We conclude from this that the receptor for IL 1 in the plasma membranes of K 562 is a transmembranous protein of 41 kDa, which possesses a tyrosine specific protein kinase activity with an autophosphorylating capacity.  

Harvard-Zitierstil: Resch, K., Martin, M., Lovett, D., Kyas, U. and Gemsa, D. (1986) The Receptor for Interleukin 1 in Plasma Membranes of the Human Leukemia Cell K 562: Biological and Biochemical Characterization, Immunobiology, 172(3-5), pp. 336-345. https://doi.org/10.1016/S0171-2985(86)80115-2

APA-Zitierstil: Resch, K., Martin, M., Lovett, D., Kyas, U., & Gemsa, D. (1986). The Receptor for Interleukin 1 in Plasma Membranes of the Human Leukemia Cell K 562: Biological and Biochemical Characterization. Immunobiology. 172(3-5), 336-345. https://doi.org/10.1016/S0171-2985(86)80115-2