Journal article

Publication year: 1986

Pages: 165-169

Journal: Immunobiology

Volume number: 171

Issue number: 3

ISSN: 0171-2985

DOI Link: https://doi.org/10.1016/S0171-2985(86)80001-8

Publisher: Elsevier

Abstract: 

An in vitro system was established to investigate the early effects of interleukin 1 (IL 1) on a clearly defined subcellular system, the plasma membrane. Murine IL 1 was obtained from the macrophage tumor line P 388 D1 and purified to apparent homogeneity. Plasma membranes from the human leukaemic tumor cell line K 562 were highly purified by differential ultracentrifugation. The monokine induced the specific phosphorylation of a 41 kDa plasma membrane protein. This could be demonstrated by incubating the plasma membranes in the presence of IL 1 and (gamma-32p)-ATP, separating the proteins by SDS-PAGE and subsequent autoradiography. A first biochemical reaction caused by IL 1 in the target organelle of the cell, the plasma membrane, was discovered, yielding a clue of how signal transmission may take place finally resulting in the stimulation of the responding cell.An in vitro system was established to investigate the early effects of interleukin 1 (IL 1) on a clearly defined subcellular system, the plasma membrane. Murine IL 1 was obtained from the macrophage tumor line P 388 D1 and purified to apparent homogeneity. Plasma membranes from the human leukaemic tumor cell line K 562 were highly purified by differential ultracentrifugation. The monokine induced the specific phosphorylation of a 41 kDa plasma membrane protein. This could be demonstrated by incubating the plasma membranes in the presence of IL 1 and (gamma-32p)-ATP, separating the proteins by SDS-PAGE and subsequent autoradiography. A first biochemical reaction caused by IL 1 in the target organelle of the cell, the plasma membrane, was discovered, yielding a clue of how signal transmission may take place finally resulting in the stimulation of the responding cell.

Harvard Citation style: Martin, M., Lovett, D. and Resch, K. (1986) Interleukin 1 Induces Specific Phosphorylation of a 41 kDa Plasma Membrane Protein from the Human Tumor Cell Line K 562, Immunobiology, 171(3), pp. 165-169. https://doi.org/10.1016/S0171-2985(86)80001-8

APA Citation style: Martin, M., Lovett, D., & Resch, K. (1986). Interleukin 1 Induces Specific Phosphorylation of a 41 kDa Plasma Membrane Protein from the Human Tumor Cell Line K 562. Immunobiology. 171(3), 165-169. https://doi.org/10.1016/S0171-2985(86)80001-8