Journal article

Publication year: 1995

Pages: 540-548

Journal: Biochemical and Biophysical Research Communications

Volume number: 216

Issue number: 2

ISSN: 0006-291X

DOI Link: https://doi.org/10.1006/bbrc.1995.2656

Publisher: Elsevier

Abstract: 
The mouse CD5 positive pre-B cell line SPGM-1 can be induced to switch its lineage commitment towards macrophage differentiation by treatment with a combination of phorbol ester and a calcium ionophore. When cultured with these reagents the pre-B cells ceased to proliferate and rapidly became adherent to plastic surfaces. This morphological change was accompanied by the loss of pre-B cell-specific surface markers, such as PB76 and most prominantly the mu heavy chain of the immunoglobulin receptor complex. In addition, the mRNA of the surrogate light chain lambda(5) disappeared while the induction of lysozyme mRNA could be detected. Differentiated SPGM-1 cells phagocytosed latex beads and showed nonspecific esterase activity. The high efficiency and speed of differentiation in this cellular system makes SPGM-1 a highly suitable model for studying the phenomenon of lineage switching during hemopoesis. (C) 1995 Academic Press, Inc.

Harvard Citation style: Spencker, T., Neumann, D., Strasser, A., Resch, K. and Martin, M. (1995) Lineage Switch of a Mouse Pre-B-Cell Line (SPMG-1) to Macrophage-like Cells After Incubation with Phorbol Ester and Calcium Ionophore, Biochemical and Biophysical Research Communications, 216(2), pp. 540-548. https://doi.org/10.1006/bbrc.1995.2656

APA Citation style: Spencker, T., Neumann, D., Strasser, A., Resch, K., & Martin, M. (1995). Lineage Switch of a Mouse Pre-B-Cell Line (SPMG-1) to Macrophage-like Cells After Incubation with Phorbol Ester and Calcium Ionophore. Biochemical and Biophysical Research Communications. 216(2), 540-548. https://doi.org/10.1006/bbrc.1995.2656