The Interleukin-1 Receptor Accessory Protein (IL-1RAcP) Is Essential for IL-1-induced Activation of Interleukin-1 Receptor-associated Kinase (IRAK) and Stress-activated Protein Kinases (SAP Kinases) - Forschungsportal der Justus-Liebig-Universität Gießen:

Journalartikel

The Interleukin-1 Receptor Accessory Protein (IL-1RAcP) Is Essential for IL-1-induced Activation of Interleukin-1 Receptor-associated Kinase (IRAK) and Stress-activated Protein Kinases (SAP Kinases)

Autorenliste: Wesche, H; Korherr, C; Kracht, M; Falk, W; Resch, K; Martin, M

Jahr der Veröffentlichung: 1997

Seiten: 7727-7731

Zeitschrift: Journal of Biological Chemistry

Bandnummer: 272

Heftnummer: 12

ISSN: 0021-9258

DOI Link: https://doi.org/10.1074/jbc.272.12.7727

Verlag: Elsevier

Abstract:
Interleukin-1 (IL-1) is a central mediator of the immune system involved in acute and chronic inflammatory responses. Although the sequences of two types of IL-1 receptors are known, the exact molecular events resulting in signal transduction and coupling to downstream signaling elements remain unclear. The recently cloned IL-1 receptor accessory protein (IL-1RAcP) has been suggested as a co-receptor molecule for IL-1RI, supported by the observation that its expression correlates to IL-1 responsiveness. We transfected the EL-4 subline D6/76 with IL-1RAcP cDNA. This cell line is an IL-1 non-responder expressing IL-1RI but lacking constitutive IL-1RAcP expression. The expression of IL-1RAcP in EL-4 D6/76 was sufficient to restore IL-1-induced activation of interleukin-1 receptor-associated kinase and of stress-activated protein kinases, translocation of the transcription factors NFkappaB and IL-1 NF to the nucleus, and induction of IL-2 mRNA synthesis. These results proved that IL-1RAcP is an indispensible molecule in the IL-1 receptor signal transduction complex, necessary to link events on the plasma membrane level to downstream signaling pathways, allowing IL-1-dependent activation of transcription factors and gene expression.

Zitierstile

Harvard-Zitierstil: Wesche, H., Korherr, C., Kracht, M., Falk, W., Resch, K. and Martin, M. (1997) The Interleukin-1 Receptor Accessory Protein (IL-1RAcP) Is Essential for IL-1-induced Activation of Interleukin-1 Receptor-associated Kinase (IRAK) and Stress-activated Protein Kinases (SAP Kinases), Journal of Biological Chemistry, 272(12), pp. 7727-7731. https://doi.org/10.1074/jbc.272.12.7727

APA-Zitierstil: Wesche, H., Korherr, C., Kracht, M., Falk, W., Resch, K., & Martin, M. (1997). The Interleukin-1 Receptor Accessory Protein (IL-1RAcP) Is Essential for IL-1-induced Activation of Interleukin-1 Receptor-associated Kinase (IRAK) and Stress-activated Protein Kinases (SAP Kinases). Journal of Biological Chemistry. 272(12), 7727-7731. https://doi.org/10.1074/jbc.272.12.7727