Journal article

Publication year: 2002

Pages: 1185-1192

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 52

Issue number: 4

ISSN: 1466-5026

eISSN: 1466-5034

DOI Link: https://doi.org/10.1099/00207713-52-4-1185

Publisher: Microbiology Society

Abstract: 
A novel cellulolytic and xylanolytic bacterium, strain MX5T, was isolated from the hindgut contents of the Australian termite Mastotermes darwiniensis (Froggatt). The isolate was a facultative anaerobe and had a Gram-positive cell-wall profile. The rod-shaped bacterium formed irregular coryneform and coccoid cells during growth. Phylogenetic analysis of the 16S rDNA provided evidence that the organism was closely related to the as-yet undescribed cellulolytic strain SR272 and the non-validly described species 'Cellulomonas pachnodae' as well as Promicromonospora citrea and Promicromonospora sukumoe. Strain MX5T was assigned to the genus Cellulosimicrobium on the basis of phylogenetic and chemotaxonomic criteria. The murein of strain MX5T contained the diamino acid lysine. N-Glycolylmuramic acid, mycolic acids and hydroxy fatty acids were absent. The major neutral sugar in the cell wall was galactose and the major quinone was menaquinone MK-9(H4). The predominant fatty acids were ai-C15:0, i-C15:0, i-C16:0 and C16:0. The G+C content of the DNA was in a range 70-72 mol%. On the basis of 16S rDNA sequence similarities and chemotaxonomic features, MX5T was clearly different from Cellulosimicrobium cellulans and other validly described species within this phylogenetic group. For this reason, a novel species is described, for which the name Cellulosimicrobium variabile sp. nov. is proposed.

Harvard Citation style: Bakalidou, A., Kämpfer, P., Berchtold, M., Kuhnigk, T., Wenzel, M. and König, H. (2002) Cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis., International Journal of Systematic and Evolutionary Microbiology, 52(4), pp. 1185-1192. https://doi.org/10.1099/00207713-52-4-1185

APA Citation style: Bakalidou, A., Kämpfer, P., Berchtold, M., Kuhnigk, T., Wenzel, M., & König, H. (2002). Cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis.. International Journal of Systematic and Evolutionary Microbiology. 52(4), 1185-1192. https://doi.org/10.1099/00207713-52-4-1185