Evaluation of the Titertek-Enterobac-Automated System (TTE-AS) for identification of members of the family Enterobacteriaceae - Research portal of Justus Liebig University Giessen:

Journal article

Evaluation of the Titertek-Enterobac-Automated System (TTE-AS) for identification of members of the family Enterobacteriaceae

Authors list: Kämpfer, P

Publication year: 1990

Pages: 164-172

Journal: Zentralblatt für Bakteriologie

Volume number: 273

Issue number: 2

ISSN: 0934-8840

DOI Link: https://doi.org/10.1016/s0934-8840(11)80244-6

Publisher: Urban & Fischer

Abstract:
The Titertek-Enterobac-Automated System (TTE-AS, Flow Laboratories, Meckenheim, FRG) was evaluated for identification of members of the family of Enterobacteriaceae using a total of 816 bacterial strains obtained from stock cultures and clinical specimens. A comparison of the results of the TTE-AS kit with conventional methods was conducted. Identifications were classified as correct, not identified (two or more taxa possible, identification score less than 80%, supplementary tests for final identification were not performed), and incorrect. Correct identification results were further subdivided according to the level of identification by species or biotype: (a) greater than or equal to 98%, (b) 90% to 97%, (c) 80% to 89%. When compared with conventional identification results, the TTE-AS system correctly identified 95.3% (778 of 816 strains), 90.0% (734 strains) belonging to category (a), 3.4% (28 strains) belonging to category (b), and 2.0% (16 strains) belonging to category (c). For 18 (2.2%) of the remaining strains, the system generated the correct identification result, but with an identification level less than 80% (low discrimination results). 11 strains (1.3%) were misidentified and the system failed to generate identification results with 9 strains (1.1%). Reactions within TTE-AS were reproducible with an estimated probability of erroneous test results of 1.7%.

Authors/Editors

- Uni.-Prof. Dr. Dr. Peter Kämpfer

Citation Styles

Harvard Citation style: Kämpfer, P. (1990) Evaluation of the Titertek-Enterobac-Automated System (TTE-AS) for identification of members of the family Enterobacteriaceae, Zentralblatt für Bakteriologie, 273(2), pp. 164-172. https://doi.org/10.1016/s0934-8840(11)80244-6

APA Citation style: Kämpfer, P. (1990). Evaluation of the Titertek-Enterobac-Automated System (TTE-AS) for identification of members of the family Enterobacteriaceae. Zentralblatt für Bakteriologie. 273(2), 164-172. https://doi.org/10.1016/s0934-8840(11)80244-6