Journal article

Publication year: 2010

Pages: 1729-1734

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 60

Issue number: 8

ISSN: 1466-5026

eISSN: 1466-5034

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijs.0.015990-0

Publisher: Microbiology Society

Abstract: 
Two Gram-stain-positive, mycelium-forming actinobacteria (strains 14-Be-013T and 02-Gi-014) were isolated from walls colonized with moulds and studied taxonomically. The isolates formed yellowish-pigmented substrate mycelium showing no fragmentation. Comparative analysis of 16S rRNA gene sequences showed that these bacteria are most closely related to genera within the family Nocardiopsaceae, but form a separate lineage within this family. Highest sequence similarities were to the type strains of Marinactinospora thermotolerans (96.0% to 14-Be-013T), Nocardiopsis dassonvillei subsp. albirubida and Nocardiopsis lucentensis (both 95.3% to 14-Be-013T). Whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid of the cell wall and no diagnostic sugars. Mycolic acids were absent. The major menaquinones were MK-10(H4), MK-11(H4) and MK-12(H2). The polar lipid profile consisted of phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown lipids. Major fatty acids iso-C16:0, anteiso-C17:0 and C18:1omega9c supported the affiliation of these isolates to the family Nocardiopsaceae. Phenotypic analysis (including chemotaxonomy) further differentiated strains 14-Be-013T and 02-Gi-014 from the most closely related members of the genera Marinactinospora and Nocardiopsis. Since the two strains form a distinct lineage in the 16S rRNA gene sequence-based phylogenetic tree, the novel genus Murinocardiopsis gen. nov. with the type species Murinocardiopsis flavida sp. nov. is proposed. The type strain of Murinocardiopsis flavida is 14-Be-013T (=DSM 45312T =CCM 7612T).

Harvard Citation style: Kämpfer, P., Schäfer, J., Lodders, N. and Martin, K. (2010) Murinocardiopsis flavida gen. nov., sp. nov., an actinomycete isolated from indoor walls., International Journal of Systematic and Evolutionary Microbiology, 60(8), pp. 1729-1734. https://doi.org/10.1099/ijs.0.015990-0

APA Citation style: Kämpfer, P., Schäfer, J., Lodders, N., & Martin, K. (2010). Murinocardiopsis flavida gen. nov., sp. nov., an actinomycete isolated from indoor walls.. International Journal of Systematic and Evolutionary Microbiology. 60(8), 1729-1734. https://doi.org/10.1099/ijs.0.015990-0