Journalartikel

Jahr der Veröffentlichung: 2010

Seiten: 967-971

Zeitschrift: Biosensors and Bioelectronics

Bandnummer: 25

Heftnummer: 5

ISSN: 0956-5663

DOI Link: https://doi.org/10.1016/j.bios.2009.04.002

Verlag: Elsevier

Abstract: 
An SPR-based sandwich immunoassay for serotyping of Salmonella is demonstrated. The Salmonella are captured on an SPR chip using polyclonal capture antibody. SPR sensorgrams are obtained for the immunoreactions of the somatic (O) and flagellar (H) surface antigens, of the captured bacteria, to their respective antibodies. The sensorgram data are compiled to determine the antigenic formula in accordance with the Kauffmann-White scheme. Salmonella Enteritidis was completely serotyped using this SPR-based method. In addition, Salmonella belonging to serogroups B, C and D were successfully assigned to their respective serogroups. Before serotyping the bacteria are grown to a concentration of 1x10(10) mL(-1). This SPR-based serotyping provides quantitative data, and thus, eliminates the possibility of false detections as encountered in the conventional slide agglutination test (SAT). This method was also proved to work with rough strains.

Harvard-Zitierstil: Mazumdar, S., Barlen, B., Kämpfer, P. and Keusgen, M. (2010) Surface plasmon resonance (SPR) as a rapid tool for serotyping of Salmonella., Biosensors and Bioelectronics, 25(5), pp. 967-971. https://doi.org/10.1016/j.bios.2009.04.002

APA-Zitierstil: Mazumdar, S., Barlen, B., Kämpfer, P., & Keusgen, M. (2010). Surface plasmon resonance (SPR) as a rapid tool for serotyping of Salmonella.. Biosensors and Bioelectronics. 25(5), 967-971. https://doi.org/10.1016/j.bios.2009.04.002