Journal article

Publication year: 2008

Pages: 361-364

Journal: Gefahrstoffe - Reinhaltung der Luft

Volume number: 68

Issue number: 9

ISSN: 0039-0771, 0949-8036

Publisher: Springer-VDI-Verl.

Abstract: 

At present, standardized procedures for the detection of microorganisms from bioaerosols are most often based on cultivation-dependent approaches. For the sampling of fungi the filtration, and for the sampling of bacteria the impingement is used, ensuring a high biological sampling efficiency for each of these groups of microorganisms. With cultivation-independent methods, biological sampling efficiency is negligible for total cell count quantification. Here, differentiation into living and dead cells is hardly feasible, whereas a high physical sampling efficiency is most important. Until now, only few publications describe a direct comparison between the physical sampling efficiency of microorganisms for both methods, filtration and impingement. In this study, twelve bioaerosol samples, sampled in parallel by filtration and impingement, were analyzed for total microorganism content. Therefore, bioaerosol samples from poultry stables were investigated and total cell counts were determined by fluorescence microscopy. The cell numbers obtained after the impingement were on average 40% lower than concentrations of the corresponding filtration samples. This is most probably due to the visible pre-separation of particles in the impingement inlet system. It has to be assumed, that using the AGI-30 impinger for total cell count determination leads to an underestimation of the concentration.

Harvard Citation style: Fallschissel, K., Kämpfer, P. and Jäckel, U. (2008) Vergleichende Messung luftgetragener Mikroorganismen mittels Impingement und Filtration in Geflügelställen, Gefahrstoffe - Reinhaltung der Luft, 68(9), pp. 361-364

APA Citation style: Fallschissel, K., Kämpfer, P., & Jäckel, U. (2008). Vergleichende Messung luftgetragener Mikroorganismen mittels Impingement und Filtration in Geflügelställen. Gefahrstoffe - Reinhaltung der Luft. 68(9), 361-364.