Journal article

Publication year: 1992

Pages: 373-377

Journal: Experimental Cell Research

Volume number: 199

Issue number: 2

ISSN: 0014-4827

DOI Link: https://doi.org/10.1016/0014-4827(92)90447-G

Publisher: Elsevier

Abstract: 
We have analyzed the assembly of the spliceosomal U4/U6 snRNP by injecting synthetic wild-type and mutant U4 RNAs into the cytoplasm of Xenopus oocytes and determining the cytoplasmic-nuclear distribution of U4 and U4/U6 snRNPs by CsCl density gradient centrifugation. Whereas the U4 snRNP was localized in both the cytoplasmic and nuclear fractions, the U4/U6 snRNP was detected exclusively in the nuclear fraction. Cytoplasmic-nuclear migration of the U4 snRNP did not depend on the stem II nor on the 5' stem-loop region of U4 RNA. Our data provide strong evidence that, following the cytoplasmic assembly of the U4 snRNP, the interaction of the U4 snRNP with U6 RNA/RNP occurs in the nucleus; furthermore, cytoplasmic-nuclear transport of the U4 snRNP is independent of U4/U6 snRNP assembly.

Harvard Citation style: Wersig, C., Guddat, U., Pieler, T. and Bindereif, A. (1992) Assembly and nuclear transport of the U4 and U4/U6 snRNPs., Experimental Cell Research, 199(2), pp. 373-377. https://doi.org/10.1016/0014-4827(92)90447-G

APA Citation style: Wersig, C., Guddat, U., Pieler, T., & Bindereif, A. (1992). Assembly and nuclear transport of the U4 and U4/U6 snRNPs.. Experimental Cell Research. 199(2), 373-377. https://doi.org/10.1016/0014-4827(92)90447-G