Journal article

Publication year: 2009

Pages: 164-170

Journal: Environmental and Molecular Mutagenesis

Volume number: 50

Issue number: 3

ISSN: 0893-6692

DOI Link: https://doi.org/10.1002/em.20470

Publisher: Wiley

Abstract: 

In this study, we used the single cell gel electrophoresis (SCGE) assay
and the micronucleus (MN) test to investigate the DNA damaging effects
and the antigenotoxic potencies of three structurally related ITCs in
human HepG2 cells. The results show that all three ITCs possess the
characteristic of a “Janus” compound, i.e., they exert both significant
genotoxicity and antigenotoxicity, depending on the concentrations used
in the test systems applied. Regression line analysis of the results
derived by SCGE analysis showed genotoxic potency of the ITCs in the
following order: 3‐methylthiopropyl ITC (MTPITC) > 4‐methylthiobutyl
ITC (MTBITC) > 5‐methylthiopentyl ITC (MTPeITC); however, this order
in genotoxic potency was not confirmed by MN analysis. Additionally, the
MN test showed significant mutagenicity of the test substances at
higher concentrations when compared with the SCGE assay. Twenty‐four
hour‐treatment of the cells with the ITCs, followed by a 1‐hr recovery
period, showed significant DNA repair in the SCGE assay at a
concentration ≥10 μM MTPITC, ≥3 μM MTBITC, and ≥0.1 μM MTPeITC,
respectively. In antigenotoxicity studies, the most effective
concentration of MTPITC and MTPeITC toward B(a)P‐induced DNA damage was
0.1 μM in both test systems. MTBITC suppressed MN formation in
B(a)P‐treated cells to the background level at a concentration of 1 μM.
The ambivalent character of the ITCs under studymust be further
clarified, especially in the possiblecontext of high dose therapeutic
applications.

Harvard Citation style: Lamy, E., Crößmann C. Saeed, A., Schreiner, P., Kotke, M. and Mersch-Sundermann, V. (2009) Three structurally homologous isothiocyanates exert “Janus” characteristics in human HepG2 cells, Environmental and Molecular Mutagenesis, 50(3), pp. 164-170. https://doi.org/10.1002/em.20470

APA Citation style: Lamy, E., Crößmann C. Saeed, A., Schreiner, P., Kotke, M., & Mersch-Sundermann, V. (2009). Three structurally homologous isothiocyanates exert “Janus” characteristics in human HepG2 cells. Environmental and Molecular Mutagenesis. 50(3), 164-170. https://doi.org/10.1002/em.20470