Initial events in the degradation of the polycistronic puf mRNA in Rhodobacter capsulatus and consequences for further processing steps - Research portal of Justus Liebig University Giessen:

Journal article

Initial events in the degradation of the polycistronic puf mRNA in Rhodobacter capsulatus and consequences for further processing steps

Authors list: Heck, C; Balzer, A; Fuhrmann, O; Klug, G

Publication year: 2000

Pages: 90-100

Journal: Molecular Microbiology

Volume number: 35

Issue number: 1

ISSN: 0950-382X

DOI Link: https://doi.org/10.1046/j.1365-2958.2000.01679.x

Publisher: Wiley

Abstract:
Individual segments of the polycistronic pufmRNA of Rhodobacter capsulatus exhibit extremely different half-lives contributing to the stoichiometry of light-harvesting and reaction centre complexes of this facultative phototrophic bacterium. While earlier investigations shed light on the processes leading to the degradation of the 2.7 kb pufBALMX mRNA and, consequently, to the formation of the highly stable 0.5 kb pufBA mRNA processing product, we have now investigated the initial events in the degradation of the highly unstable 3.2 kb pufQBALMX primary transcript. Sequence modifications of two putative RNase E recognition sites within the pufQ coding region provide strong evidence that RNase E-mediated cleavage of a sequence at the 3' end of pufQ is involved in rate-limiting cleavage of the primary pufQBALMX transcript in vivo. The putative RNase E recognition sequence at the 5' end of pufQ is cleaved in vitro but does not contribute to rate-limiting cleavage in vivo. Analysis of the decay of pufmRNA segments transcribed from wildtype and mutated pufDNA sequences in R. capsulatus and Escherichia coli reveal that RNase E-mediated cleavage within the pufQ mRNA sequence also affects the stability of the 0.5 kb pufBA processing product. These findings demonstrate that the stability of a certain mRNA segment depends on the pathway of processing of its precursor molecule.

Authors/Editors

- Prof. Dr. Gabriele Klug

Citation Styles

Harvard Citation style: Heck, C., Balzer, A., Fuhrmann, O. and Klug, G. (2000) Initial events in the degradation of the polycistronic puf mRNA in Rhodobacter capsulatus and consequences for further processing steps, Molecular Microbiology, 35(1), pp. 90-100. https://doi.org/10.1046/j.1365-2958.2000.01679.x

APA Citation style: Heck, C., Balzer, A., Fuhrmann, O., & Klug, G. (2000). Initial events in the degradation of the polycistronic puf mRNA in Rhodobacter capsulatus and consequences for further processing steps. Molecular Microbiology. 35(1), 90-100. https://doi.org/10.1046/j.1365-2958.2000.01679.x