Journal article

Publication year: 1998

Pages: 297-305

Journal: Molecular and General Genetics MGG

Volume number: 258

Issue number: 3

ISSN: 0026-8925

DOI Link: https://doi.org/10.1007/s004380050734

Publisher: Springer Verlag (Germany)

Abstract: 
The puf and puc operons of Rhodabacter capsulatus, which encode pigment binding proteins, are regulated by oxygen tension. Strain IR4 which carries a point mutation in the himA gene for integration host factor (IHF) shows a delayed increase in the amount of photosynthetic complexes after reduction of oxygen tension when compared to the isogenic wild-type strain. This delay in the formation of photosynthetic complexes correlates with a delay in increasing the level of transcription of the puf and puc operons, whereas the transcription of the bchD genes is not affected by IHF. Several IHF binding sites can be identified upstream of the puf and puc transcriptional starts by in vitro footprint analysis. Our results suggest that IHF facilitates the binding of other trans-acting factors, e.g. RegA, which in turn stimulate transcription of the puf and puc genes under low oxygen tension.

Harvard Citation style: Kirndorfer, M., Jager, A. and Klug, G. (1998) Integration host factor affects the oxygen-regulated expression of photosynthesis genes in Rhodobacter capsulatus, Molecular and General Genetics MGG, 258(3), pp. 297-305. https://doi.org/10.1007/s004380050734

APA Citation style: Kirndorfer, M., Jager, A., & Klug, G. (1998). Integration host factor affects the oxygen-regulated expression of photosynthesis genes in Rhodobacter capsulatus. Molecular and General Genetics MGG. 258(3), 297-305. https://doi.org/10.1007/s004380050734