Journal article

Publication year: 2004

Pages: 4563-4575

Journal: Nucleic Acids Research

Volume number: 32

Issue number: 15

ISSN: 0305-1048

Open access status: Green

DOI Link: https://doi.org/10.1093/nar/gkh794

Publisher: Oxford University Press

Abstract: 
The expression of many genes of facultatively photosynthetic bacteria of the genus Rhodobacter is controlled by the oxygen tension. Among these are the genes of the puf and puc operons, which encode proteins of the photosynthetic apparatus. Previous results revealed that thioredoxins are involved in the regulated expression of these operons, but it remained unsolved as to the mechanisms by which thioredoxins affect puf and puc expression. Here we show that reduced TrxA of Rhodobacter capsulatus and Rhodobacter sphaeroides and oxidized TrxC of R.capsulatus interact with DNA gyrase and alter its DNA supercoiling activity. While TrxA enhances supercoiling, TrxC exerts a negative effect on this activity. Furthermore, inhibition of gyrase activity strongly reduces puf and puc expression. Our results reveal a new signaling pathway by which oxygen can affect the expression of bacterial genes.

Harvard Citation style: Li, K., Pasternak, C., Hartig, E., Haberzettl, K., Maxwell, A. and Klug, G. (2004) Thioredoxin can influence gene expression by affecting gyrase activity, Nucleic Acids Research, 32(15), pp. 4563-4575. https://doi.org/10.1093/nar/gkh794

APA Citation style: Li, K., Pasternak, C., Hartig, E., Haberzettl, K., Maxwell, A., & Klug, G. (2004). Thioredoxin can influence gene expression by affecting gyrase activity. Nucleic Acids Research. 32(15), 4563-4575. https://doi.org/10.1093/nar/gkh794