Journal article

Publication year: 2001

Pages: 307-313

Journal: Journal of Industrial Microbiology and Biotechnology

Volume number: 27

Issue number: 5

ISSN: 1367-5435

DOI Link: https://doi.org/10.1038/sj.jim.7000144

Publisher: Oxford University Press

Abstract: 
The gene of Orf5 (SolR) of Clostridium acetobutylicum DSM 792 was subcloned and overexpressed in Escherichia coli. The protein was purified with Ni-NTA agarose and used for DNA binding assays. No DNA binding of Orf5 to regions upstream of the sol operon from C. acetobutylicum was observed. Overexpression of Orf5 in C. acetobutylicum led to a change in the organism's pattern of glycosylated exoproteins. The Orf5 protein was localized in the cell membrane fraction and to a small extent in the supernatant medium. Based on these results Orf5 (SolR) appears not to act as a transcriptional repressor in C. acetobutylicum, but instead may be an enzyme involved in glycosylation or deglycosylation.

Harvard Citation style: Thormann, K. and Dürre, P. (2001) Orf5/SolR: a transcriptional repressor of the sol operon of Clostridium acetobutylicum?, Journal of Industrial Microbiology and Biotechnology, 27(5), pp. 307-313. https://doi.org/10.1038/sj.jim.7000144

APA Citation style: Thormann, K., & Dürre, P. (2001). Orf5/SolR: a transcriptional repressor of the sol operon of Clostridium acetobutylicum?. Journal of Industrial Microbiology and Biotechnology. 27(5), 307-313. https://doi.org/10.1038/sj.jim.7000144