Journal article

Publication year: 2015

Pages: 727-742

Journal: Molecular Microbiology

Volume number: 98

Issue number: 4

ISSN: 0950-382X

Open access status: Bronze

DOI Link: https://doi.org/10.1111/mmi.13152

Publisher: Wiley

Abstract: 
Spatiotemporal regulation of cell polarity plays a role in many fundamental processes in bacteria and often relies on landmark' proteins which recruit the corresponding clients to their designated position. Here, we explored the localization of two multi-protein complexes, the polar flagellar motor and the chemotaxis array, in Shewanella putrefaciensCN-32. We demonstrate that polar positioning of the flagellar system, but not of the chemotaxis system, depends on the GTPase FlhF. In contrast, the chemotaxis array is recruited by a transmembrane protein which we identified as the functional ortholog of Vibrio choleraeHubP. Mediated by its periplasmic N-terminal LysM domain, SpHubP exhibits an FlhF-independent localization pattern during cell cycle similar to its Vibrio counterpart and also has a role in proper chromosome segregation. In addition, while not affecting flagellar positioning, SpHubP is crucial for normal flagellar function and is involved in type IV pili-mediated twitching motility. We hypothesize that a group of HubP/FimV homologs, characterized by a rather conserved N-terminal periplasmic section required for polar targeting and a highly variable acidic cytoplasmic part, primarily mediating recruitment of client proteins, serves as polar markers in various bacterial species with respect to different cellular functions.

Harvard Citation style: Rossmann, F., Brenzinger, S., Knauer, C., Dörrich, A., Bubendorfer, S., Ruppert, U., et al. (2015) The role of FlhF and HubP as polar landmark proteins in Shewanella putrefaciensCN-32, Molecular Microbiology, 98(4), pp. 727-742. https://doi.org/10.1111/mmi.13152

APA Citation style: Rossmann, F., Brenzinger, S., Knauer, C., Dörrich, A., Bubendorfer, S., Ruppert, U., Bange, G., & Thormann, K. (2015). The role of FlhF and HubP as polar landmark proteins in Shewanella putrefaciensCN-32. Molecular Microbiology. 98(4), 727-742. https://doi.org/10.1111/mmi.13152