Contribution in an anthology

Appeared in: The Bacterial Flagellum : Methods and Protocols

Editor list: Tohru, M; Keiichi, N

Publication year: 2017

Pages: 285-295

ISBN: 978-1-4939-6926-5

eISBN: 978-1-4939-6927-2

DOI Link: https://doi.org/10.1007/978-1-4939-6927-2_23

Edition: 1. Auflage

Title of series: Methods in Molecular Biology

Number in series: 1593

Abstract: 

The stator is an eminent component of the flagellar motor and determines
a number of the motor’s properties, such as the rotation-energizing
coupling ion (H⁺ or Na⁺) or the torque that can be
generated. The stator consists of several units located in the
cytoplasmic membrane surrounding the flagellar drive shaft. Studies on
flagellar motors of several bacterial species have provided evidence
that the number as well as the retention time of stators coupled to the
motor is highly dynamic and depends on the environmental conditions.
Notably, numerous species possess more than a single distinct set of
stators. It is likely that the presence of different stator units
enables these bacteria to adjust the flagellar motor properties and
function to meet the environmental requirements. One of these species is
Shewanella oneidensis MR-1 that is equipped with a single polar flagellum and two stator units, the Na⁺-dependent PomAB and the H⁺-dependent
MotAB. Here, we describe a method to determine stator dynamics by
fluorescence microscopy, demonstrating how bacteria can change the
composition of an intricate molecular machine according to environmental
conditions.

Harvard Citation style: Brenzinger, S. and Thormann, T. (2017) Dynamics in the Dual Fuel Flagellar Motor of Shewanella oneidensis MR-1, in Tohru, M. and Keiichi, N. (eds.) The Bacterial Flagellum : Methods and Protocols. 1. Auflage. New York: Springer New York - Humana Press, pp. 285-295. https://doi.org/10.1007/978-1-4939-6927-2_23

APA Citation style: Brenzinger, S., & Thormann, T. (2017). Dynamics in the Dual Fuel Flagellar Motor of Shewanella oneidensis MR-1. In Tohru, M., & Keiichi, N. (Eds.), The Bacterial Flagellum : Methods and Protocols (1. Auflage, pp. 285-295). Springer New York - Humana Press. https://doi.org/10.1007/978-1-4939-6927-2_23