Journalartikel

Jahr der Veröffentlichung: 2002

Seiten: 475-484

Zeitschrift: The Journal of Physiology

Bandnummer: 545

Heftnummer: 2

ISSN: 0022-3751

DOI Link: https://doi.org/10.1113/jphysiol.2002.031914

Verlag: Wiley

Abstract: 
We report on responses of olfactory receptor neurons (ORNs) upon application of amino acids and forskolin using a novel slice preparation of the olfactory epithelium of Xenopus laevis tadpoles. Responses were measured using the patch-clamp technique. Both amino acids and forskolin proved to be potent stimuli. Interestingly, a number of ORNs that responded to amino acids did not respond to forskolin. This suggests that some amino acids activate transduction pathways other than the well-known cAMP-mediated one. The differential processing of cAMP-mediated stimuli on the one hand and amino acid stimuli on the other was further elucidated by calcium-imaging of olfactory bulb neurons using a novel nose-olfactory bulb preparation of Xenopus laevis tadpoles. The projection pattern of amino acid-sensitive ORNs to olfactory bulb neurons differed markedly from the projection pattern of forskolin-sensitive ORNs. Olfactory bulb neurons activated by amino acids were located laterally compared to those activated by forskolin, and only a small proportion responded to both stimuli. The ensemble of neurons activated by forskolin was also activated by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and the membrane-permeant cAMP analogue 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate (pCPT-cAMP). We therefore conclude that sensory transduction of a number of amino acids is cAMP independent, and amino acid- and cAMP-mediated responses are processed differentially at the level of the olfactory bulb.

Harvard-Zitierstil: Manzini, I., Rössler, W. and Schild, D. (2002) cAMP-independent responses of olfactory neurons in Xenopus laevis tadpoles and their projection onto olfactory bulb neurons, The Journal of Physiology, 545(2), pp. 475-484. https://doi.org/10.1113/jphysiol.2002.031914

APA-Zitierstil: Manzini, I., Rössler, W., & Schild, D. (2002). cAMP-independent responses of olfactory neurons in Xenopus laevis tadpoles and their projection onto olfactory bulb neurons. The Journal of Physiology. 545(2), 475-484. https://doi.org/10.1113/jphysiol.2002.031914