Journal article

Publication year: 2001

Pages: 459-462

Journal: FEBS Letters

Volume number: 508

Issue number: 3

ISSN: 0014-5793

DOI Link: https://doi.org/10.1016/S0014-5793(01)02988-X

Publisher: Wiley

Abstract: 
We have successfully co-expressed two genes from the bilin biosynthetic pathway of Synechocystis together with cyanobacterial phytochrome 1 (Cph1) from the same organism to produce holophytochrome in Escherichia coli. Heme oxygenase was used to convert host heme to biliverdin IX alpha which was then reduced to phycocyanobilin via phycocyanobilin:ferredoxin oxidoreductase, presumably with the aid of host ferredoxin. In this host environment Cph1 apophytochrome was able to autoassemble with the phycocyanobilin in vivo to form fully photoreversible holophytochrome. The system can be used as a tool for further genetic studies of phytochrome function and signal transduction as well as providing an excellent source of holophytochrome for physicochemical studies. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Harvard Citation style: Landgraf, F., Forreiter, C., Pico, A., Lamparter, T. and Hughes, J. (2001) Recombinant holophytochrome in Escherichia coli, FEBS Letters, 508(3), pp. 459-462. https://doi.org/10.1016/S0014-5793(01)02988-X

APA Citation style: Landgraf, F., Forreiter, C., Pico, A., Lamparter, T., & Hughes, J. (2001). Recombinant holophytochrome in Escherichia coli. FEBS Letters. 508(3), 459-462. https://doi.org/10.1016/S0014-5793(01)02988-X