Journal article

Publication year: 1998

Pages: 394-400

Journal: Journal of Plant Physiology

Volume number: 153

Issue number: 3-4

ISSN: 0176-1617

DOI Link: https://doi.org/10.1016/S0176-1617(98)80166-1

Publisher: Elsevier

Abstract: 
This paper describes somatic fusion of protoplasts in the moss Ceratodon purpureus, specifically of the phototropic mutants ptr103 and ptr116. Phototropism of Ceratodon filaments is phytochrome mediated. The ptr116 mutant is thought to carry a lesion in the biosynthetic pathway of the phytochrome chromophore; this mutant grows negatively gravitropically in continuous unilateral red light. The ptr103 mutation is thought to effect phytochrome signal transduction: the phenotype of this mutant is clearly distinguishable from wildtype and ptr116 because filaments grow in random directions in continuous unilateral red. Following protoplasting and PEG-mediated protoplast fusion with ptr103 and ptr116, lines with a wildtype-like phenotype were identified, propagated and tested for genome size and phytochrome physiology. The 1C genome size of Ceratodon is estimated to lie between 240 and 270 Mbp, based on comparisons with Arabidopsis thaliana (genome size 100 Mbp). The genome size of one line that arose from a fusion experiment, Ptr103(+)ptr116, was shown by flow cytometry to be ca. twice that of the wildtype. With respect to phytochrome spectral activity, phototropism and regulation of chlorophyll synthesis, ptr103(+)ptr116 was similar to the wildtype. These data are consistent with ptr103(+)ptr116 being a bona fide somatic fusion product in which each mutant parent complements a recessive genetic lesion of the other.

Harvard Citation style: Lamparter, T., Brucker, G., Esch, H., Hughes, J., Meister, A. and Hartmann, E. (1998) Somatic hybridisation with aphototropic mutants of the moss Ceratodon purpureus: Genome size, phytochrome photoreversibility, tip-cell phototropism and chlorophyll regulation, Journal of Plant Physiology, 153(3-4), pp. 394-400. https://doi.org/10.1016/S0176-1617(98)80166-1

APA Citation style: Lamparter, T., Brucker, G., Esch, H., Hughes, J., Meister, A., & Hartmann, E. (1998). Somatic hybridisation with aphototropic mutants of the moss Ceratodon purpureus: Genome size, phytochrome photoreversibility, tip-cell phototropism and chlorophyll regulation. Journal of Plant Physiology. 153(3-4), 394-400. https://doi.org/10.1016/S0176-1617(98)80166-1