Journal article

Publication year: 1987

Pages: 355-363

Journal: Plant Molecular Biology

Volume number: 9

Issue number: 4

ISSN: 0167-4412

DOI Link: https://doi.org/10.1007/BF00014910

Publisher: Springer

Abstract: 

Transcript levels of two plastid genes were investigated during early seedling development of mustard (Sinapis alba L.) until 96 h after sowing. The two genes, which are closely linked and have the same polarity, are the psbA gene encoding the M_r 32–35000 herbicide-binding Q_B-protein of photosystem II and the trnK gene encoding plastid tRNA^Lys
(UUU) and potentially an intron-derived maturase-related protein. By
using Northern and dot blot hybridization techniques with sensitive RNA
probes, the 1.2 kb psbA transcript
was found to be present in low amounts during the initial phase of seed
germination. Thereafter, it increases in concentration both in light-
and dark-grown seedlings until approximately 48 h after sowing. A
further increase in psbA transcript
concentration during the subsequent phase until 96 h was observed in
light-grown, but not in dark-grown seedlings. The 2.8 kb trnK transcript is one to two orders of magnitude less abundant than the psbA
transcript throughout the time period investigated. The concentration
of this transcript is light-independent and shows a transient peak level
at around 48 h, i.e. at the onset of light-enhanced accumulation of the
psbA transcript.

Harvard Citation style: Hughes, J., Neuhaus, H. and Link, G. (1987) Transcript levels of two adjacent chloroplast genes during mustard (Sinapis alba L.) seedling development are under differential temporal and light control, Plant Molecular Biology, 9(4), pp. 355-363. https://doi.org/10.1007/BF00014910

APA Citation style: Hughes, J., Neuhaus, H., & Link, G. (1987). Transcript levels of two adjacent chloroplast genes during mustard (Sinapis alba L.) seedling development are under differential temporal and light control. Plant Molecular Biology. 9(4), 355-363. https://doi.org/10.1007/BF00014910