Journal article

Publication year: 1993

Pages: 902-910

Journal: Rapid Communications in Mass Spectrometry

Volume number: 7

Issue number: 10

ISSN: 0951-4198

DOI Link: https://doi.org/10.1002/rcm.1290071010

Publisher: Wiley

Abstract: 
In matrix-assisted laser desorption ionization (MALDI) a large fraction of analyte ions undergo post-source decay (PSD) during flight in the field-free drift path. By means of a modified two-stage reflectron, product ion time-of-flight spectra of medium-sized linear peptides (up to 2800 u) were recorded, containing full sequence information. Precision, accuracy and mass resolution of fragment ions were almost as good as obtained in high-energy collisionally activated dissociation (CAD) studies performed in four-sector instruments. Instrumental sensitivity was better by at least one order of magnitude. In reflectron time-of-flight mass spectrometry (RETOF-MS) the cleavage pattern of PSD products is different from that obtained by high-energy and low-energy CAD. Activation mechanisms of PSD were found to be largely determined by collisional events (ion/neutral) occurring in the acceleration field during early plume expansion. Future potentials of PSD analysis after MALDI are discussed.

Harvard Citation style: Kaufmann, R., Spengler, B. and Lützenkirchen (1993) Mass spectrometric sequencing of linear peptides by product‐ion analysis in a reflectron time‐of‐flight mass spectrometer using matrix‐assisted laser desorption ionization, Rapid Communications in Mass Spectrometry, 7(10), pp. 902-910. https://doi.org/10.1002/rcm.1290071010

APA Citation style: Kaufmann, R., Spengler, B., & Lützenkirchen (1993). Mass spectrometric sequencing of linear peptides by product‐ion analysis in a reflectron time‐of‐flight mass spectrometer using matrix‐assisted laser desorption ionization. Rapid Communications in Mass Spectrometry. 7(10), 902-910. https://doi.org/10.1002/rcm.1290071010