Sequencing of a branched peptide using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry - Forschungsportal der Justus-Liebig-Universität Gießen:

Journalartikel

Sequencing of a branched peptide using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Autorenliste: Fournier, I; Chaurand, P; Bolbach, G; Lützenkirchen, F; Spengler, B; Tabet, JC

Jahr der Veröffentlichung: 2000

Seiten: 1425-1433

Zeitschrift: Journal of Mass Spectrometry

Bandnummer: 35

Heftnummer: 12

ISSN: 1076-5174

DOI Link: https://doi.org/10.1002/1096-9888(200012)35:12<1425::AID-JMS77>3.0.CO;2-C

Verlag: Wiley

Abstract:
Chemical degradation methods combined with matrix-assisted laser desorption/ionization time-of-flight ((MALDI-TOF) and post-source decay (PSD)-MALDI reflex TOF mass spectrometry (MS) were used to determine the sequence of a peptide branched on to a known peptide backbone. This study was applied to a branched peptide model (derivative of substance P), The branched peptide mimics a digest of a membrane receptor on to which a derivative of substance P was photochemically linked. Chemical degradation based on N-terminal ladder sequencing in combination with MALDI-TOF-MS gave only partial sequence information. Although single PSD mass spectra still remain difficult to interpret unambiguously, PSD-MALDI-TOF-MS was combined with on-target acetylation and H-D exchange to give a better and successful approach to the unambiguous determination of the complete amino acid side-chain sequence. This study shows the capability of MALDI-TOF-MS to help in characterizing ligand-receptor interactions.

Zitierstile

Harvard-Zitierstil: Fournier, I., Chaurand, P., Bolbach, G., Lützenkirchen, F., Spengler, B. and Tabet, J. (2000) Sequencing of a branched peptide using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Journal of Mass Spectrometry, 35(12), pp. 1425-1433. https://doi.org/10.1002/1096-9888(200012)35:12<1425::AID-JMS77>3.0.CO;2-C

APA-Zitierstil: Fournier, I., Chaurand, P., Bolbach, G., Lützenkirchen, F., Spengler, B., & Tabet, J. (2000). Sequencing of a branched peptide using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Journal of Mass Spectrometry. 35(12), 1425-1433. https://doi.org/10.1002/1096-9888(200012)35:12<1425::AID-JMS77>3.0.CO;2-C