Journal article
Authors list: Rühl, M; Kües, U
Publication year: 2009
Pages: 241-253
Journal: Current trends in biotechnology and pharmacy
Volume number: 3
Issue number: 3
Publisher: Association of Biotechnology and Pharmacy (ABAP)
In this study, an image analysis system forfungal pellets was developed
Abstract:
using thecommercial software system analySIS¨ and theprotocol was
evaluated in morphological studiesof pellet formation in submerged
cultures of thebasidiomycete Coprinopsis cinerea. Pellets wereanalysed
on large scale (60 to 130 pellets perimage, 225 to 400 pellets per
culture).Morphologies of pellets were characterised bythe parameters
grey value, pellet area, convexity,shape factor, sphericity and pellet
diameter.Threshold values were defined for all parametersfor object
filtering. By application of theparameter filter, aggregated hyphal
fragmentspresent in larger amounts particularly in culturesgrown at
higher temperature (370C) could beclearly distinguished in image
analysis fromcompact pellets. At a lower growth temperature(250C), there
was little background of loosehyphal material, fungal pellets were
regularlyshaped and the pellets remained constant in shapeand size over a
longer cultivation period.
Citation Styles
Harvard Citation style: Rühl, M. and Kües, U. (2009) Automated image analysis to observe pellet morphology in liquid cultures of filamentous fungi such as the basidiomycete Coprinopsis cinerea, Current trends in biotechnology and pharmacy, 3(3), pp. 241-253. http://abap.co.in/automated-image-analysis-observe-pellet-morphology-liquid-cultures-filamentous-fungi-such-basidiomyc
APA Citation style: Rühl, M., & Kües, U. (2009). Automated image analysis to observe pellet morphology in liquid cultures of filamentous fungi such as the basidiomycete Coprinopsis cinerea. Current trends in biotechnology and pharmacy. 3(3), 241-253. http://abap.co.in/automated-image-analysis-observe-pellet-morphology-liquid-cultures-filamentous-fungi-such-basidiomyc
