Journal article

Publication year: 2009

Pages: 2339-2342

Journal: FEBS Letters

Volume number: 583

Issue number: 14

ISSN: 1873-3468

DOI Link: https://doi.org/10.1016/j.febslet.2009.06.026

Publisher: Wiley

Abstract: 
Sinorhizobium meliloti harbours genes encoding orthologs of ribonuclease (RNase) E and RNase J, the principle endoribonucleases in Escherichia coli and Bacillus subtilis, respectively. To analyse the role of RNase J in S. meliloti, RNA from a mutant with miniTn5-insertion in the RNase J-encoding gene was compared to the wild-type and a difference in the length of the 5.8S-like ribosomal RNA (rRNA) was observed. Complementation of the mutant, Northern blotting and primer extension revealed that RNase J is necessary for the 5'-end maturation of 16S rRNA and of the two 23S rRNA fragments, but not of 5S rRNA. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Harvard Citation style: Madhugiri, R. and Evguenieva-Hackenberg, E. (2009) RNase J is involved in the 5 '-end maturation of 16S rRNA and 23S rRNA in Sinorhizobium meliloti, FEBS Letters, 583(14), pp. 2339-2342. https://doi.org/10.1016/j.febslet.2009.06.026

APA Citation style: Madhugiri, R., & Evguenieva-Hackenberg, E. (2009). RNase J is involved in the 5 '-end maturation of 16S rRNA and 23S rRNA in Sinorhizobium meliloti. FEBS Letters. 583(14), 2339-2342. https://doi.org/10.1016/j.febslet.2009.06.026