Sammelbandbeitrag

Erschienen in: Archaea : molecular and cellular biology

Herausgeberliste: Cavicchioli, R

Jahr der Veröffentlichung: 2007

Seiten: 158-174

ISBN: 978-1-55581-391-8

Abstract: 

Most organisms contain between 40 and 50 different transfer RNA (tRNA)
molecules that read one or more of the 61 or 62 different sense codons
through specific codon-anticodon interactions and position the
appropriate amino acid for insertion into the growing polypeptide chain.
Additional complexity in the tRNA processing and modification pathway
occurs in cases where archaeal tRNA genes contain an intron. The chapter
describes introns in archaeal transcripts. The genes for ribosomal RNA
(rrn) are located in operons in the archaeal genome and are transcribed
to produce multicistronic precursor RNAs. Two early studies used
nuclease protection and primer extension assays to define the
intermediates generated during processing of the primary rRNA transcript
from two canonical rrn operons: the single-rrn operon in Halobacterium salinarum and the canonical rrnA operon in Haloarcula marismortui.
The proportion of modified nucleotides in tRNA can approach 50% or
more. The two most frequent modifications in RNA are ribose methylation
and the pseudouridylation. In an attempt to distinguish between these
two alternatives, actinomycin D was used to inhibit transcription and
Northern hybridization was used to follow the decay of the various mRNA
fragments. There was a strong correlation between fragment length and
stability: the shorter the fragment, the longer the half-life. This
correlation was used to argue that the transcript fragments are
generated by endonucleotic cleavage rather than premature transcription
termination, and that the distal sequences released following cleavage
are selectively degraded.

Harvard-Zitierstil: Klug, G., Evguenieva-Hackenberg, E., Omer, A., Dennis, P. and Marchfelder, A. (2007) RNA processing, in Cavicchioli, R. (ed.) Archaea : molecular and cellular biology. Washington, DC: ASM Press, pp. 158-174

APA-Zitierstil: Klug, G., Evguenieva-Hackenberg, E., Omer, A., Dennis, P., & Marchfelder, A. (2007). RNA processing. In Cavicchioli, R. (Ed.), Archaea : molecular and cellular biology (pp. 158-174). ASM Press.