Journal article

Publication year: 1993

Pages: 204-208

Journal: FEBS Letters

Volume number: 326

Issue number: 1-3

ISSN: 0014-5793

DOI Link: https://doi.org/10.1016/0014-5793(93)81791-W

Publisher: Wiley

Abstract: 
IL-1 receptor heterogeneity in murine lymphocytes was investigated by cross-linking to [I-125]IL-1alpha and competition with IL-1 receptor antagonist, and the molecular identity of the IL-1 receptors was identified with PCR using primers specific for type I and type II IL-1 receptors. The thymoma cell line EI4 6.1 exhibits exclusively the 80 kDa receptors which proved to be the type I receptor according to PCR analysis. In the pre-B cell fine 70Z/3, predominantly a 60 kDa type II receptor but also a trace of type I receptor can be identified by PCR. The Th2 cell line D10N expresses both types of IL-1 receptors in equivalent amounts according to cross-linking experiments and PCR. The proliferative response of D10N cells to IL-1 is inhibited by IL-1 ra which according to cross-linking affects the binding to the type I receptor only. It is concluded that coexpression of both types of IL-1 receptors might be a characteristic of murine Th2 cells and that their growth-dependence on IL-1 is mediated by the type I receptor.

Harvard Citation style: Brigelius-Flohe, R., McCarthy, J., Resch, K., Szamel, M. and Martin, M. (1993) Coexpression of type I and type II IL‐1 receptors in the murine T helper 2 cell line D10N, FEBS Letters, 326(1-3), pp. 204-208. https://doi.org/10.1016/0014-5793(93)81791-W

APA Citation style: Brigelius-Flohe, R., McCarthy, J., Resch, K., Szamel, M., & Martin, M. (1993). Coexpression of type I and type II IL‐1 receptors in the murine T helper 2 cell line D10N. FEBS Letters. 326(1-3), 204-208. https://doi.org/10.1016/0014-5793(93)81791-W