Journal article

Publication year: 1994

Pages: 1566-1571

Journal: European Journal of Immunology

Volume number: 24

Issue number: 7

ISSN: 0014-2980

DOI Link: https://doi.org/10.1002/eji.1830240717

Publisher: Wiley

Abstract: 
We have investigated the possibility of a protein kinase participating in the signal transduction mechanisms of the interleukin-1 (IL-1) type I receptor (IL-1RI). Our data show that a protein kinase was co-precipitated with the IL-1 RI from the two murine T helper cell lines D 10N and EL-4. The kinase activity was detected in an in vitro kinase assay performed with the immune beads in the presence of exogenous substrates. IL-1 treatment of the cells resulted in a rapid activation of this protein kinase in a concentration-dependent manner. Both forms of IL-1, IL-1 alpha and IL-1 beta, induced this kinase activity, whereas the IL-1 receptor antagonist (IL-1ra) was inactive. In excess IL-1ra competitively antagonized IL-1 stimulation. In the in vitro kinase assay the exogenous substrates myelin basic protein and histone H-1 were phosphorylated, whereas casein or heat-shock protein HSP27 were not accepted, reflecting a certain selectivity of this protein kinase. The IL-1RI co-precipitable protein kinase showed a serine/threonine specificity and was inhibited by staurosporine, but not by inhibitors specific for protein tyrosine kinases or protein kinase C. These results show that a serine/threonine protein kinase directly interacts with the IL-1RI at the plasma membrane level of T helper cells forming a novel type of IL-1 inducible signaling complex. This protein kinase may resemble the link coupling the plasma membrane IL-1 receptor to cytosolic downstream elements in the IL-1 signaling pathway.

Harvard Citation style: Martin, M., Böl, G., Eriksson, A., Resch, K. and Brigelius-Flohe, R. (1994) Interleukin‐1‐induced activation of a protein kinase co‐precipitating with the type I interleukin‐1 receptor in T cells, European Journal of Immunology, 24(7), pp. 1566-1571. https://doi.org/10.1002/eji.1830240717

APA Citation style: Martin, M., Böl, G., Eriksson, A., Resch, K., & Brigelius-Flohe, R. (1994). Interleukin‐1‐induced activation of a protein kinase co‐precipitating with the type I interleukin‐1 receptor in T cells. European Journal of Immunology. 24(7), 1566-1571. https://doi.org/10.1002/eji.1830240717