Journal article

Publication year: 1996

Pages: 50-59

Journal: Medizinische Klinik

Volume number: 91

Issue number: Supplement 3

ISSN: 0723-5003

Publisher: Springer Verlag (Germany)

Abstract: 

BACKGROUND: The clinical use of ex vivo expanded hematopoietic progenitor cells is currently explored.

MATERIAL AND METHODS: In this study the culturing of G-CSF mobilized and purified CD34+ blood cells was investigated. The interleukins IL-1 beta, IL-3 and IL-6 (each at a dose of 300 U/ml) and stem cell factor (25 ng/ml) without or with erythropoietin (1 U/ml) were used. Cells of 10 healthy sibling donors and 10 patients with solid tumors were incubated under small-scale (n = 15, 2 ml) and large-scale (n = 7, 50 ml) culture conditions at 37 degrees C for 5 and 4 weeks, respectively. The cell density was adjusted to about 1 x 10(5) cells/ml.

RESULTS: The nucleated cell counts increased approximately 7-fold and 10- to 70-fold after 1 and 2 weeks of incubation. Numbers of CD34+ cells doubled to triplicated within this time interval, without any significant changes in their clonogenicity (CFU-GM and BFU-E output). Thereafter a depletion of the CD34+ cell pool was noticed. However the numbers of CD34+/CD38(-)- or CD34+/ HLA-DR(-)- cells were reduced to a lesser extent. The expanded cells generated predominantly myeloid and almost no lymphoid cells. More glycophorin-A+ cells were produced when erythropoietin was added. Replacement of non-human additives with heat-inactivated autologous plasma had no influence on cell growth. Almost no proliferation was obtained with a 10-fold higher cell density (1.7 x 10(6)/ml in 100 ml), but the cells maintained their viability for 13 to 16 days.

CONCLUSIONS: This study suggests, that the chosen culture conditions might be feasible for a large-scale ex vivo expansion of hematopoietic progenitor cells for clinical application. The impact of the ex vivo generated cells on hematopoietic regeneration after chemotherapy is currently under clinical investigation.

Harvard Citation style: Arseniev, L., Battmer, K., Stucki, A., Südmeyer, I., Kadar, J., Martin, M., et al. (1996) Ex-vivo Vermehrung von hämatopoetischen Progenitorzellen für die klinische Anwendung, Medizinische Klinik, 91(Supplement 3), pp. 50-59

APA Citation style: Arseniev, L., Battmer, K., Stucki, A., Südmeyer, I., Kadar, J., Martin, M., & Link, H. (1996). Ex-vivo Vermehrung von hämatopoetischen Progenitorzellen für die klinische Anwendung. Medizinische Klinik. 91(Supplement 3), 50-59.