Journal article

Publication year: 1997

Pages: 580-589

Journal: Cell Death & Differentiation

Volume number: 4

Issue number: 7

ISSN: 1350-9047

DOI Link: https://doi.org/10.1038/sj.cdd.4400279

Publisher: Springer Nature [academic journals on nature.com]

Abstract: 
The CD5(+) mouse pre-B cell line SPGM-1 is able to undergo lineage conversion towards a macrophage like cell type, Although one of the kappa-light chain alleles is rearranged in V-J kappa 4 configuration, no protein is expressed nor could the expression be induced, Infection with and expression of the human bcl-2 gene protected SPGM-1 cells from apoptosis, allowing the rearrangement of their second kappa-allele in V-J kappa 5 configuration, mRNA transcripts of the V-J kappa regions were detected only in SPGM-1 x bcl-2 cells, but not in SPGM-1, while kappa-light chain protein was not found in any of the cell lines. The myeloid differentiation potential of SPGM-1 cells was not affected by overexpression of the human bcl-2 gene. Upon appropriate stimulation, SPGM-1 x bcl-2 cells became enlarged, adhered to the plastic surfaces and lost their immunoglobulin mu heavy chain expression.

Harvard Citation style: Neumann, D., Strasser, A., Wesche, H., Resch, K. and Martin, M. (1997) Overexpression of the bcl-2 oncogene in the mouse pre-B cell line SPGM-1 protects from apoptosis, but does not affect blocked B-lineage differentiation and lineage switch towards macrophage like cells, Cell Death & Differentiation, 4(7), pp. 580-589. https://doi.org/10.1038/sj.cdd.4400279

APA Citation style: Neumann, D., Strasser, A., Wesche, H., Resch, K., & Martin, M. (1997). Overexpression of the bcl-2 oncogene in the mouse pre-B cell line SPGM-1 protects from apoptosis, but does not affect blocked B-lineage differentiation and lineage switch towards macrophage like cells. Cell Death & Differentiation. 4(7), 580-589. https://doi.org/10.1038/sj.cdd.4400279