Effects of overexpression of IL-1 receptor-associated kinase on NF kappa B activation, IL-2 production and stress-activated protein kinases in the murine T cell line EL4 - Research portal of Justus Liebig University Giessen:

Journal article

Effects of overexpression of IL-1 receptor-associated kinase on NF kappa B activation, IL-2 production and stress-activated protein kinases in the murine T cell line EL4

Authors list: Knop, J; Wesche, H; Lang, D; Martin, MU

Publication year: 1998

Pages: 3100-3109

Journal: European Journal of Immunology

Volume number: 28

Issue number: 10

ISSN: 0014-2980

DOI Link: https://doi.org/10.1002/(SICI)1521-4141(199810)28:10<3100::AID-IMMU3100>3.0.CO;2-7

Publisher: Wiley

Abstract:
The association and activation of the IL-1 receptor-associated protein kinase (IRAK) to the IL-1 receptor complex is one of the earliest events detectable in IL-1 signal transduction. We generated permanent clones of the murine T cell line EL4 6.1 overexpressing human (h)IRAK to evaluate the role of this kinase in IL-1 signaling. Overexpression of hIRAK enhanced IL-1-stimulated activation of the transcription factor NF kappa B, whereas a truncated form (N-IRAK) specifically inhibited IL-1-dependent NF kappa B activity. In clones stably overexpressing hIRAK a weak constitutive activation of NF kappa B correlated with a low basal IL-2 production which was enhanced in an IL-1-dependent manner. Compared to the parental cell line the dose-response curve of IL-1-induced IL-2 production was shifted in both potency and efficacy. These results demonstrate that IRAK directly triggers NF kappa B-mediated gene expression in EL4 cells. Qualitatively different effects were observed for the IL-1-induced activation of stress-activated protein (SAP) kinases: permanent overexpression of IRAK did not affect the dose dependence but prolonged the kinetics of IL-1-induced activation of SAP kinases, suggesting that this signaling branch may be regulated by distinct mechanisms.

Citation Styles

Harvard Citation style: Knop, J., Wesche, H., Lang, D. and Martin, M. (1998) Effects of overexpression of IL-1 receptor-associated kinase on NF kappa B activation, IL-2 production and stress-activated protein kinases in the murine T cell line EL4, European Journal of Immunology, 28(10), pp. 3100-3109. https://doi.org/10.1002/(SICI)1521-4141(199810)28:10<3100::AID-IMMU3100>3.0.CO;2-7

APA Citation style: Knop, J., Wesche, H., Lang, D., & Martin, M. (1998). Effects of overexpression of IL-1 receptor-associated kinase on NF kappa B activation, IL-2 production and stress-activated protein kinases in the murine T cell line EL4. European Journal of Immunology. 28(10), 3100-3109. https://doi.org/10.1002/(SICI)1521-4141(199810)28:10<3100::AID-IMMU3100>3.0.CO;2-7