Journal article

Publication year: 2001

Pages: 635-642

Journal: Journal of Interferon and Cytokine Research

Volume number: 21

Issue number: 8

ISSN: 1079-9907

DOI Link: https://doi.org/10.1089/10799900152547902

Publisher: Mary Ann Liebert

Abstract: 
Interleukin-18 (IL-18) is a proinflammatory cytokine that plays an important role in innate immunity and specific Th1 immune responses. During infection, IL-18 is produced by activated macrophages and primarily induces interferon-gamma (IFN-gamma) production in synergy with IL-12 in primed T cells and natural killer (NK) cells. IL-18 exerts its function by binding to a specific receptor complex (IL-18R) consisting of two subunits, the IL-18R alpha chain and the IL-18R beta chain. Two individual mechanisms for synergism between IL-12 and IL-18 have been reported. First, IL-12-induced expression of the IL-18 receptor complex was described. Second, IL-18 and IL-12 together induced expression of target genes on a transcriptional level. In this study, we show that freshly isolated thymocytes from BALB/c mice constitutively expressed mRNA for the IL-18R alpha chain but not for the IL-18R beta chain. These thymocytes were unresponsive to IL-18. Treatment with a combination of concanavalinA (ConA) plus IL-12 did not affect expression of the IL-18R alpha chain but strongly induced expression for IL-18R beta mRNA, rendering these thymocytes highly responsive to IL-18 treatment. Thus, the synergistic effects of IL-12 and IL-18 on BALB/c mouse thymocytes resulted from the induction and regulation of the IL-18R beta chain and not the IL-18R alpha chain.

Harvard Citation style: Neumann, D. and Martin, M. (2001) Interleukin-12 upregulates the IL-18R beta chain in BALB/c thymocytes, Journal of Interferon and Cytokine Research, 21(8), pp. 635-642. https://doi.org/10.1089/10799900152547902

APA Citation style: Neumann, D., & Martin, M. (2001). Interleukin-12 upregulates the IL-18R beta chain in BALB/c thymocytes. Journal of Interferon and Cytokine Research. 21(8), 635-642. https://doi.org/10.1089/10799900152547902