Journalartikel

Jahr der Veröffentlichung: 2005

Seiten: 2202-2211

Zeitschrift: Arthritis & rheumatism

Bandnummer: 52

Heftnummer: 7

ISSN: 0004-3591

DOI Link: https://doi.org/10.1002/art.21108

Verlag: Wiley

Abstract: 
Objective. To discern the mode of interleukin-1 (IL-1) inhibition of soluble IL-1 receptor accessory protein (sIL-1RAcP) by comparison with IL-1 receptor antagonist (IL-1Ra) in arthritis.Methods. Adenoviral vectors encoding either sIL-1RAcP or IL-1Ra were administered systemically before onset of collagen-induced arthritis in DBA/1 mice. Anti-bovine type 11 collagen IgG and IL-6 were quantified in serum. Proliferative response of splenic T cells was determined in the presence of sIL-1RAcP or IL-1Ra. The effect on IL-1 inhibition of recombinant sIL-1RAcP and IL-1Ra was further examined in vitro, using NF-KB luciferase reporter cell lines. Quantitative polymerase chain reaction was used to determine the relative messenger RNA expression of the IL-1 receptors.Results. Adenoviral overexpression of both sIL-1RAcP and IL-1Ra resulted in amelioration of the collagen-induced arthritis. Both IL-1 antagonists reduced the circulating levels of antigen-specific IgG2a antibodies, but only IL-1Ra was able to inhibit lymphocyte proliferation. By using purified lymphocyte populations derived from NF-kappa B reporter mice, we showed that sIL-1RAcP inhibits IL-1-induced NF-kappa B activity in B cells but not T cells, whereas IL-1Ra inhibited IL-1 on both cell types. A study in a panel of NF-kappa B luciferase reporter cells showed that the sIL-1RAcP inhibits IL-1 signaling on cells expressing either low levels of membrane IL-IRAcP or high levels of IL-1RII.Conclusion. We show that the sIL-1RAcP ameliorated experimental arthritis without affecting T cell immunity, in contrast to IL-1Ra. Our results provide data in support of receptor competition by sIL-1RAcP as an explanation for the different mode of IL-1 antagonism in comparison with IL-1Ra.

Harvard-Zitierstil: Smeets, R., Joosten, L., Arntz, O., Bennink, M., Takahashi, N., Carlsen, H., et al. (2005) Soluble interleukin-1 receptor accessory protein ameliorates collagen-induced arthritis by a different mode of action from that of interleukin-1 receptor antagonist, Arthritis & rheumatism, 52(7), pp. 2202-2211. https://doi.org/10.1002/art.21108

APA-Zitierstil: Smeets, R., Joosten, L., Arntz, O., Bennink, M., Takahashi, N., Carlsen, H., Martin, M., van den Berg, W., & van de Loo, F. (2005). Soluble interleukin-1 receptor accessory protein ameliorates collagen-induced arthritis by a different mode of action from that of interleukin-1 receptor antagonist. Arthritis & rheumatism. 52(7), 2202-2211. https://doi.org/10.1002/art.21108