Sammelbandbeitrag

Erschienen in: Environmental Responses in Plants : Methods and Protocols

Herausgeberliste: Duque, P

Jahr der Veröffentlichung: 2016

Seiten: 21-28

ISBN: 978-1-4939-3354-9

eISBN: 978-1-4939-3356-3

DOI Link: https://doi.org/10.1007/978-1-4939-3356-3_3

Serientitel: Methods in Molecular Biology

Serienzählung: 1398

Abstract: 

Plants utilize light as sole energy source. To maximize light capture they are able to detect the light direction and orient themselves towards the light source. This phototropic response is mediated by the plant blue light photoreceptors phototropin1 and 2 (phot1 and phot2). Although fully differentiated plants also exhibit this response it can be best observed in etiolated seedlings. Differences in light between the illuminated and shaded site of a seedling stem lead to changes in the auxin-distribution, resulting in cell elongation on the shaded site. Since phototropism connects light perception, signaling, and auxin transport, it is of great interest to analyze this response with a fast and simple method.
Here we describe a method to analyze the phototropic response of Arabidopsis seedlings. With numerous mutants available, its fast germination and its small size Arabidopsis is well suited for this analysis. Different genotypes can be simultaneously probed in less than a week.

Harvard-Zitierstil: Zeidler, M. (2016) Physiological Analysis of Phototropic Responses in Arabidopsis, in Duque, P. (ed.) Environmental Responses in Plants : Methods and Protocols. New York: Springer - Humana Press, pp. 21-28. https://doi.org/10.1007/978-1-4939-3356-3_3

APA-Zitierstil: Zeidler, M. (2016). Physiological Analysis of Phototropic Responses in Arabidopsis. In Duque, P. (Ed.), Environmental Responses in Plants : Methods and Protocols (pp. 21-28). Springer - Humana Press. https://doi.org/10.1007/978-1-4939-3356-3_3