Journal article

Publication year: 2003

Pages: 885-891

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 53

Issue number: 3

ISSN: 1466-5026

eISSN: 1466-5034

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijs.0.02357-0

Publisher: Microbiology Society

Abstract: 
A group of seven sucrose-negative Aeromonas strains (referred to as group Au) isolated from the internal organs of septicaemic farmed frogs (Rana rugulosa) in Thailand was subjected to a polyphasic taxonomic study including fluorescent amplified fragment length polymorphism (FAFLP) and ERIC-PCR fingerprinting, 16S rDNA sequencing, microplate DNA-DNA hybridizations and extensive phenotypic characterization. Comparison of FAFLP and ERIC-PCR fingerprints indicated that the group Au isolates belonged to the species Aeromonas hydrophila DNA hybridization group (HG) 1 in which they represent a genotypic subgroup closely affiliated to A. hydrophila subsp. hydrophila and subsp. dhakensis. One representative of the Au group exhibited > or = 99.0% 16S rDNA sequence similarity with the type strains of the two A. hydrophila subspecies. DNA-DNA hybridization with type and reference strains of all known Aeromonas taxa revealed that the Au group represented a homogeneous taxon that exhibited the highest relatedness with members of the two A. hydrophila subspecies, ranging from 75 to 93%. Phenotypic characterization on the basis of 152 features further revealed that the Au group isolates differed from A. hydrophila subsp. hydrophila or subsp. dhakensis in a total of 13 biochemical properties. Of these, assimilation of L-glycine and isobutyrate as sole carbon source, acid production from salicin and D-sucrose, and aesculin hydrolysis were of diagnostic value. From the results of this study, it can be concluded that the Aeromonas frog isolates of the Au group represent a new subspecies of A. hydrophila, for which the name Aeromonas hydrophila subsp. ranae subsp. nov. is proposed. Its type strain is Au-1D12(T) (=LMG 19707(T) = CCUG 46211(T)).

Harvard Citation style: Huys, G., Pearson, M., Kämpfer, P., Denys, R., Cnockaert, M., Inglis, V., et al. (2003) Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand., International Journal of Systematic and Evolutionary Microbiology, 53(3), pp. 885-891. https://doi.org/10.1099/ijs.0.02357-0

APA Citation style: Huys, G., Pearson, M., Kämpfer, P., Denys, R., Cnockaert, M., Inglis, V., & Swings, J. (2003). Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand.. International Journal of Systematic and Evolutionary Microbiology. 53(3), 885-891. https://doi.org/10.1099/ijs.0.02357-0