Journalartikel

Jahr der Veröffentlichung: 1999

Seiten: 25-35

Zeitschrift: International Journal of Systematic Bacteriology

Bandnummer: 49

Heftnummer: 1

ISSN: 0020-7713

Open Access Status: Bronze

DOI Link: https://doi.org/10.1099/00207713-49-1-25

Verlag: Microbioloy Society

Abstract: 
Scotochromogenic mycobacterial isolates from water-damaged parts of indoor building materials of a children's day care centre represented a phenetically and genetically distinct group of strains. A 16S rDNA dendrogram (1243 bp) showed that the closest species to the new strain MA112/96T was Mycobacterium abscessus. Phylogenetic and phenetic analyses (100 characteristics) grouped the new isolates with M. abscessus, Mycobacterium vaccae, Mycobacterium aurum and Mycobacterium austroafricanum. Ribotyping with Pvull restriction distinguished the 5 isolates from the other 12 most closely related species by the major bands at 6.5-7 kb and 13-15 kb. The cell morphology of the new isolates was typical of mycobacteria, electron microscopy revealed a triple-layered cell wall with an irregular electron-dense outer layer. They grew at 10-37 degrees C, with no growth at 45 degrees C in 5 d. The gene encoding the secreted 32 kDa protein, specific to mycobacteria, was detected by PCR. The main whole-cell fatty acids were characterized by high tuberculostearic acid 10Me-C18:0 (17% at 28 degrees C), which increased with increasing growth temperature (22% at 37 degrees C). The other main fatty acids were C18:1 cis9 and C16:0 (21-20% each), followed by, C17:1 cis9 (14%), C16:1 cis10 (8%) and also a high amount of C20 alcohol (9%). alpha-Mycolic acids, keto-mycolates and wax esters were present (C60-C90), MK-9(H2) (90%) and MK-8(H2) were the main menaquinones. The cellular phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidyl inositolmannosides and diphosphatidylglycerol. Polyamine content was low. G+C content was 72.9 mol%. The new isolates are proposed as a new species, Mycobacterium murale sp. nov. The type strain is MA112/96T (= DSM 44340T).

Harvard-Zitierstil: Vuorio, R., Andersson, M., Rainey, F., Kroppenstedt, R., Kämpfer, P., Busse, H., et al. (1999) A new rapidly growing mycobacterial species, Mycobacterium murale sp. nov., isolated from the indoor walls of a children's day care centre., International Journal of Systematic Bacteriology, 49(1), pp. 25-35. https://doi.org/10.1099/00207713-49-1-25

APA-Zitierstil: Vuorio, R., Andersson, M., Rainey, F., Kroppenstedt, R., Kämpfer, P., Busse, H., Viljanen, M., & Salkinoja-Salonen, M. (1999). A new rapidly growing mycobacterial species, Mycobacterium murale sp. nov., isolated from the indoor walls of a children's day care centre.. International Journal of Systematic Bacteriology. 49(1), 25-35. https://doi.org/10.1099/00207713-49-1-25