Journal article

Publication year: 2015

Pages: 4093-4099

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 65

Issue number: 11

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.000547

Publisher: Microbiology Society

Abstract: 
A beige-pigmented, oxidase-negative bacterial strain (JM-458T), isolated from a rhizosphere sample, was studied using a polyphasic taxonomic approach. Cells of the isolate were rod-shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequence of strain JM-458T with sequences of the type strains of closely related species of the genus Enterobacter showed that it shared highest sequence similarity with Enterobacter mori (98.7 %), Enterobacter hormaechei (98.3 %), Enterobacter cloacae subsp. dissolvens, Enterobacter ludwigii and Enterobacter asburiae (all 98.2 %). 16S rRNA gene sequence similarities to all other Enterobacter species were below 98 %. Multilocus sequence analysis based on concatenated partial rpoB, gyrB, infB and atpD gene sequences showed a clear distinction of strain JM-458T from its closest related type strains. The fatty acid profile of the strain consisted of C16 : 0, C17 : 0 cyclo, iso-C15 : 0 2-OH/C16 : 1ω7c and C18 : 1ω7c as major components. DNA-DNA hybridizations between strain JM-458T and the type strains of E. mori, E. hormaechei and E. ludwigii resulted in relatedness values of 29 % (reciprocal 25 %), 24 % (reciprocal 43 %) and 16 % (reciprocal 17 %), respectively. DNA-DNA hybridization results together with multilocus sequence analysis results and differential biochemical and chemotaxonomic properties showed that strain JM-458T represents a novel species of the genus Enterobacter, for which the name Enterobacter muelleri sp. nov. is proposed. The type strain is JM-458T ( = DSM 29346T = CIP 110826T = LMG 28480T = CCM 8546T).

Harvard Citation style: Kämpfer, P., McInroy, J. and Glaeser, S. (2015) Enterobacter muelleri sp. nov., isolated from the rhizosphere of Zea mays., International Journal of Systematic and Evolutionary Microbiology, 65(11), pp. 4093-4099. https://doi.org/10.1099/ijsem.0.000547

APA Citation style: Kämpfer, P., McInroy, J., & Glaeser, S. (2015). Enterobacter muelleri sp. nov., isolated from the rhizosphere of Zea mays.. International Journal of Systematic and Evolutionary Microbiology. 65(11), 4093-4099. https://doi.org/10.1099/ijsem.0.000547