Journal article

Publication year: 2013

Pages: 2630-2636

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 63

Issue number: 7

ISSN: 1466-5026

DOI Link: https://doi.org/10.1099/ijs.0.048280-0

Publisher: Microbiology Society

Abstract: 
A yellow-pigmented, Gram-negative rod, designated FNE08-86(T), was isolated from subsurface water of the humic-matter-rich and almost-neutral north-east basin of the experimentally divided bog lake Grosse Fuchskuhle (Brandenburg, Germany). Analysis of the nearly full-length 16S rRNA gene sequence showed the highest 16S rRNA gene sequence similarity with Novosphingobium rosa IAM 14222(T) (96.3 %). Sequence similarities with all other members of the genus Novosphingobium species were <96 %, but phylogenetic tree construction clearly showed the placement of strain FNE08-86(T) within the genus Novosphingobium. The predominant fatty acids were C18 : 1ω7c and C16 : 0, and only a single 2-hydroxy fatty acid, C14 : 0 2-OH, was detected. The polar lipid profile revealed phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine as major compounds, with smaller amounts of sphingoglycolipid, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and several unidentified lipids. In the quinone system ubiquinone Q-10 was predominant and in the polyamine pattern spermidine was predominant. Characterization by genotypic, chemotaxonomic and phenotypic analysis indicated that strain FNE08-86(T) represents a novel species of the genus Novosphingobium, for which we propose the name Novosphingobium aquaticum sp. nov. (type strain FNE08-86(T) = DSM 25088(T) = CCM 7983(T)).

Harvard Citation style: Glaeser, S., Bolte, K., Busse, H., Kämpfer, P., Grossart, H. and Glaeser, J. (2013) Novosphingobium aquaticum sp. nov., isolated from the humic-matter-rich bog lake Grosse Fuchskuhle., International Journal of Systematic and Evolutionary Microbiology, 63(7), pp. 2630-2636. https://doi.org/10.1099/ijs.0.048280-0

APA Citation style: Glaeser, S., Bolte, K., Busse, H., Kämpfer, P., Grossart, H., & Glaeser, J. (2013). Novosphingobium aquaticum sp. nov., isolated from the humic-matter-rich bog lake Grosse Fuchskuhle.. International Journal of Systematic and Evolutionary Microbiology. 63(7), 2630-2636. https://doi.org/10.1099/ijs.0.048280-0