Journalartikel

Jahr der Veröffentlichung: 2002

Seiten: 473-483

Zeitschrift: International Journal of Systematic and Evolutionary Microbiology

Bandnummer: 52

Heftnummer: 2

ISSN: 1466-5026

eISSN: 1466-5034

Open Access Status: Bronze

DOI Link: https://doi.org/10.1099/00207713-52-2-473

Verlag: Microbiology Society

Abstract: 
Four aerobic, gram-negative bacterial strains isolated from kaolin slurry used in the production of paper were subjected to a polyphasic analysis and characterization to determine their taxonomic position. Analysis of the 16S rDNA sequences of the four strains revealed that they represent a new lineage within the gamma-Proteobacteria, related to the genera Xanthomonas, Pseudoxanthomonas, Stenotrophomonas, Luteimonas, Xylella and Rhodanobacter. Analysis of the quinone system, the polyamines, the fatty acids and the polar lipids revealed a combination of characteristics that is unique and not described for the phylogenetic relatives. The four strains contain a ubiquinone Q-8, spermidine as the major polyamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the predominant polar lipids, and a fatty acid profile with predominantly iso-branched fatty acids. The G+C content of the genomic DNA was determined to be within the narrow range 67.1-68.7 mol%. Determination of DNA relatedness, as well as riboprint band patterns and amplified fragment length polymorphism profiles, clearly demonstrated that the four strains are members of a single species. Antibiotic-susceptibility patterns were identical for the four strains. Although showing a high degree of similarites in physiological and biochemical patterns, each of the four strains could be distinguished from the others on the basis of a few biochemical characteristics. On the basis of the estimates of phylogenetic relationships derived from the 16S rDNA sequence analyses, the observed chemotaxonomic characteristics and other phenotypic traits, a new genus, Thermomonas gen. nov., and species, Thermomonas haemolytica sp. nov., are proposed for the strains A50-7-3T (= DSM 13605T = LMG 19653T), B 50-7-1 (= DSM 13598 = LMG 19655), D50-7-1 (= DSM 13610 = LMG 19656) and B50-8-1 (= DSM 13599 = LMG 19654), with strain A50-7-3T as the type strain.

Harvard-Zitierstil: Busse, H., Kämpfer, P., Moore, E., Nuutinen, J., Tsitko, I., Denner, E., et al. (2002) Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry., International Journal of Systematic and Evolutionary Microbiology, 52(2), pp. 473-483. https://doi.org/10.1099/00207713-52-2-473

APA-Zitierstil: Busse, H., Kämpfer, P., Moore, E., Nuutinen, J., Tsitko, I., Denner, E., Vauterin, L., Valens, M., Rosselló-Mora, R., & Salkinoja-Salonen, M. (2002). Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry.. International Journal of Systematic and Evolutionary Microbiology. 52(2), 473-483. https://doi.org/10.1099/00207713-52-2-473