Journal article

Publication year: 2008

Pages: 365-368

Journal: Gefahrstoffe - Reinhaltung der Luft

Volume number: 68

Issue number: 9

ISSN: 0039-0771, 0949-8036

Publisher: Springer-VDI-Verl.

Abstract: 

Realtime PCR is a molecular biological method offering a high potential for the quantification of specific genes. For the detection of exposure to Salmonella cells in animal stables the DNA extraction method and subsequent realtime PCR was analysed for applicability. Recovery rates of the target organism in mixed cultures and in bioaerosol samples of a poultry stable averaged at 23%. Furthermore, a positive correlation (r(2) = 0.99) between cell number and measured fluorescence was found. Compared to the cultivation based approach, where 3.3 x 10(2) CFU Salmonella per m(3) air were found, by means of the established protocol an exposure of 1.6 x 10(5) Salmonella cells per m(3) air were detected in a broiler chicken stable. These results show, that under certain conditions the number of Salmonella cells in the inhaled air can be high and reach with about 2% a quite high proportion at the total cell number of microorganisms. Hence, these results show the applicability of realtime PCR for analysis and quantification of microorganisms in bioaerosols.

Harvard Citation style: Jäckel, U., Fallschissel, K. and Kämpfer, P. (2008) Untersuchung von Bioaerosolen aus Tierställen mittels Realtime PCR, Gefahrstoffe - Reinhaltung der Luft, 68(9), pp. 365-368

APA Citation style: Jäckel, U., Fallschissel, K., & Kämpfer, P. (2008). Untersuchung von Bioaerosolen aus Tierställen mittels Realtime PCR. Gefahrstoffe - Reinhaltung der Luft. 68(9), 365-368.