Contribution in an anthology

Appeared in: Virus-Induced Gene Silencing : Methods and Protocols

Editor list: Becker, A

Publication year: 2013

Pages: 83-98

ISBN: 978-1627032773

eISBN: 978-1-62703-278-0

DOI Link: https://doi.org/10.1007/978-1-62703-278-0_7

Title of series: Methods in Molecular Biology

Number in series: 975

Abstract: 
Eschscholzia californica
(California poppy), a member of the basal eudicot family of the
Papaveraceae, is an important species to study alkaloid biosynthesis and
the effect of alkaloids on plant metabolism. More recently, it has also
been developed as a model system to study the evolution of plant
morphogenesis. While progress has been made towards establishing methods
for generating genetically modified cell culture lines, transcriptome
data and gene expression analysis, the stable transformation and
subsequent regeneration of transgenic plants has proven extremely time
consuming and difficult. Here, we describe in detail a method to
transiently down regulate expression of a target gene by virus-induced
gene silencing (VIGS) and the subsequent analysis of the VIGS treated
plants. VIGS in E. californica
allows for the study of gene function within 2 to 3 weeks after
inoculation, and the method proves very efficient, enabling the rapid
analysis of gene functions.

Harvard Citation style: Tekleyphans, D., Lange, S. and Becker, A. (2013) Virus-Induced Gene Silencing of the Alkaloid-Producing Basal Eudicot Model Plant Eschscholzia californica (California Poppy), in Becker, A. (ed.) Virus-Induced Gene Silencing : Methods and Protocols. Totowa: Humana Press, pp. 83-98. https://doi.org/10.1007/978-1-62703-278-0_7

APA Citation style: Tekleyphans, D., Lange, S., & Becker, A. (2013). Virus-Induced Gene Silencing of the Alkaloid-Producing Basal Eudicot Model Plant Eschscholzia californica (California Poppy). In Becker, A. (Ed.), Virus-Induced Gene Silencing : Methods and Protocols (pp. 83-98). Humana Press. https://doi.org/10.1007/978-1-62703-278-0_7