Journal article

Publication year: 2011

Pages: 7482-7490

Journal: Organic & Biomolecular Chemistry

Volume number: 9

Issue number: 21

Open access status: Green

DOI Link: https://doi.org/10.1039/C1OB05929G

Publisher: Royal Society of Chemistry

Abstract: 

We prepared novel C5-modified triphosphates and phosphoramidites with a diamondoid functionally linked to the nucleobase. Using primer extension experiments with different length templates we investigated whether the modified triphosphates were enzymatically incorporated into DNA and whether they were further extended. We found that all three modified nucleotides can be incorporated into DNA using a single-nucleotide incorporation experiment, but only partially using two templates that demand for multiple incorporation of the modified nucleotides. The modified phosphoramidites were introduced into oligonucleotides utilizing DNA synthesizer technology. The occurring oligonucleotide structures were examined by circular dichroism (CD) and melting temperature (T-m) measurements and were found to adapt similar helix conformations as their unmodified counterparts.

Harvard Citation style: Wang, Y., Tkachenko, B., Schreiner, P. and Marx, A. (2011) Diamondoid-modified DNA, Organic and Biomolecular Chemistry, 9(21), pp. 7482-7490. https://doi.org/10.1039/C1OB05929G

APA Citation style: Wang, Y., Tkachenko, B., Schreiner, P., & Marx, A. (2011). Diamondoid-modified DNA. Organic and Biomolecular Chemistry. 9(21), 7482-7490. https://doi.org/10.1039/C1OB05929G